THE EFFECT OF Tinospora crispa ON ANTI OXIDANT STATUS IN STREPTOZOTOCIN INDUCED DIABETIC RATS

Sarah Firdausa, Maung Maung Cho, Khin Maung Maung, Nilar Aung, Norsidah Kuzaifah

Abstract


Diabetes is a leading cause of cellular oxidative stress which can lead to toxic effects on structure and function of organs, including β-cell in the pancreas. Islet cells of pancreas are among the tissues that have the lowest level of an antioxidant defense. In a chronic hyperglycemic state, a number of reactive oxygen species accumulate in an excess amount and cause chronic oxidative stress in the islet cell. This study aimed to investigate the effect of Tinospora crispa aqueous extract (TC) on oxidant status in streptozotocin (STZ)-induced diabetic rats. Fifty mg/kgbw of STZ was intraperitoneally induced to Sprague-Dawley rats. The animals were divided into five groups (n=6); normal (N), normal TC treated (NT), diabetic (D), diabetic TC treated (DT), and diabetic treated with Vitamin E (DE), respectively. Treatment was delivered for 4 weeks and blood sample were withdrawn for parameters measurement. The data among groups were compared using one way ANOVA test, while paired t-test was used to analyse the differences between data of pre and post treatment. In normal groups, the antioxidant and oxidative stress level of N group did not differ noticeably with NT group. In diabetic groups, the antioxidant status in DT rats showed that TC might prevent a decrease of superoxide dismutase level which was reduced in other diabetic groups. It also increased the glutathione peroxidase enzymes in animals TC treated (NT and DT). Oxidative stress level (lipid hydroperoxide and isoprostane) went up substantially in group D and no significant changes in other groups. These results showed that TC might protect diabetic subjects from reactive oxygen species generation by enhanced ant-oxidant enzymes level.


Full Text:

PDF

References


Al-alusi NT, Kadir FA, Ismail S, and Abdullah MA. 2010. In vitro interaction of combined plants: Tinospora crispa and Swietenia mahagoni against Methicillin-resistant Staphylococcus aureus (MRSA). African J Microbiol Res 4(21): 2309-2312.

Amom, Bahari H, Isemaail S, Ismail NA, Md Shah Z, and Shah MSA. 2009. Nutritional Composition, Antioxidant Ability and Flavonoid Content of Tinospora crispa stem. Adv in Nat Appl Sci 3(1): 88-94.

Bennett PH and Knowler WC. 2005. Definition, Diagnosis, and Classification of Diabetes Mellitus and Glucose Homeostasis. In C. R. Kahn, G. C. Weir, G. L. King, A. M. Jacobson, A. C. Moses & R. J. Smith (Eds.), Joslin's Diabetes Mellitus (14th ed., pp. 331-339): Lippincott Williams & Wilkins.

Davi G, Ciabattoni G, Consoli A, Mezzetti A, Falco A, Santarone S, Costantini F. 1999. In vivo formation of 8-iso-prostaglandin F2α and platelet activation in diabetes mellitus: effects of improved metabolic control and vitamin E supplementation. Circulation 99(2): 224-229.

Devaraj S, Hirany SV, Burk RF, and Jialal I. 2001. Divergence between LDL Oxidative Susceptibility and Urinary F2-Isoprostanes as Measures of Oxidative Stress in Type 2 Diabetes. Clin Chem 47(11): 1974-1979.

Freeman BA and Crapo JD. 1982. Biology of disease: free radicals and tissue injury. Laboratory investigation; J Tech Met Pathol 47(5): 412.

Halliwell B. 1994. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 344(8924): 721-724.

Hodgson EK and Fridovich I. 1975. Interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide. Inactivation of the enzyme. Biochemistry 14(24): 5294-5299.

Ibahim MJ, Wan-Nor IWMZ. 2011. Anti-proliperative and antioxidant effects of Tinospora crispa (Batawali). Biomedical Res 22(1): 57-62.

Martin-Gallan P, Carrascosa A, Gussinye M, and Dominguez C. 2003. Biomarkers of diabetes-associated oxidative stress and antioxidant status in young diabetic patients with or without subclinical complications. Free Radic Biol Med 34(12): 1563-1574.

McCord JM, Keele BB, and Fridovich I. 1971. An enzyme-based theory of obligate anaerobiosis: the physiological function of superoxide dismutase. Proceedings of the National Academy of Sciences, 68(5), 1024.

Montero A, Munger KA, Khan RZ, Valdivielso JM, Morrow JD, Guasch A, Badr KF. 2000. F2-isoprostanes mediate high glucose-induced TGF-&bgr; synthesis and glomerular proteinuria in experimental type I diabetes. Kidney International 58(5): 1963-1972.

Patrono C and Fitz Gerald GA. 1997. Isoprostanes: potential markers of oxidant stress in atherothrombotic disease. Arterioscler Thromb Vasc Biol 17(11): 2309-2315.

Pratico D. 1999. F2-isoprostanes: sensitive and specific non-invasive indices of lipid peroxidation in vivo. Atherosclerosis 147(1): 1-10.

Roberts LJ and Morrow JD. 1997. The generation and actions of isoprostanes. Biochimica et biophysica acta, L. Lipids and lipid metabolism, 1345(2):121-135.

Robertson RP. 2004. Chronic Oxidative Stress as a Central Mechanism for Glucose Toxicity in Pancreatic Islet Beta Cells in Diabetes. J Biol Chem, 279(41):42351-42354. doi: 10.1074/jbc.R400019200

Sozmen EY, Sozmen B, Delen Y, and Onat T. 2001. Catalase/superoxide dismutase (SOD) and catalase/paraoxonase (PON) ratios may implicate poor glycemic control. Arch Med Res 32(4): 283-287.

Sriwanthana B, and Chavalittumrong P. 2000. In vitro effect of Tinospora crispa on cell-mediated immunity. Warasan Krom Witthayasat Kan Phaet, 42(3): 193-201.

Sun F, Iwaguchi K, Shudo R, Nagaki Y, Tanaka K, Ikeda K, Kojo S. 1999. Change in tissue concentrations of lipid hydroperoxides, vitamin C and vitamin E in rats with streptozotocin-induced diabetes. Clini Sci 96:185-190.

Wohaieb SA and Godin DV. 1987. Alterations in free radical tissue-defense mechanisms in streptozocin-induced diabetes in rat. Effects of insulin treatment. Diabetes 36(9): 1014.

Wolff, S. P., Jiang, Z. Y., & Hunt, J. V. (1991). Protein glycation and oxidative stress in diabetes mellitus and ageing. Free Rad Biol Med 10(5): 339-352.

Zaridah, M. Z., Idid, S. Z., Wan Omar, A., & Khozirah, S. (2001). In vitro antifilarial effects of three plant species against adult worms of subperiodic Brugia malayi. J Ethnopharmacol 78(1):79-84.


Refbacks

  • There are currently no refbacks.