TOXICITY SUBCHRONIC ETHANOLIC EXTRACT OF MALAKA (Phyllanthus emblica) LEAVES ON LIVER FUNCTION OF MICE (Mus musculus): REVIEWED FROM SERUM BILIRUBIN LEVEL

Nuzul Asmilia, Yudha Fahrimal, Mahdi Abrar, Rinidar Rinidar

Abstract


The aim of this research was to investigate the subchronic toxicity of ethanolic extract of malacca leaves (Phyllanthus emblica) on liver function by analyzing bilirubin level in mice blood (Mus musculus). Sixteen male mice weighing between 25-30 grams were used in this study. Ethanolic extract of malacca leaves was given orally once a day for 21 consecutive days. Mice were randomly divided into 4 groups of 4 mice each. Control group (P0) was given distilled water; group P1, P2, and P3 were administered with ethanolic extract of malacca leaves with the dose of 300 mg/kg bb, 600 mg/kg bb, and 1200 mg/kg bb, respectively. Blood samples were taken on day 21 after treatment to measure mice bilirubin level. The results showed that blood bilirubin level of mice in P0, P1, P2, and P3 were 1.4; 1.4; 1.4, and 1.4mg/dl respectively. In conclusion, the administration of ethanolic extract of malacca leaves with repeated doses for 21 days does not have negative effect on mice liver.


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References


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