DNA Isolation on Captive Sumatran Elephant in Elephant Training Center, Way Kambas National Park: A First Step towards Its ID Card

Elly L. Rustiati, Priyambodo Priyambodo, Siti Asiyah, Dedi Candra, Diah E. Anggraini, Elizabeth D. Krismuniarti, Eko Agus Srihanto, Liza Angeliya, Nuning Nurcahyani, Enny Saswiyanti

Abstract


Elephant Training Center (ETC) Way Kambas National Park (WKNP) was built to support human-elephant
mitigation conflict. The small population of captive sumatran elephant in ETC WKNP need a comprehensive
strategy in order to maintain the genetic variation of each individual and avoid inbreeding drive. Currently, genetic
studies have opened new field studies in ecology, included conservation ecology. Patterns in variation of population
has been investigated by molecular method supporting species conservation effort. The captive sumatran elephant’s
ID Card is a necessary in database building, which included morphology, health status, and genetic profile. Genetic
profile in each ID Card was filled by cytogenetic and molecular profile for RADP result, that initiated with DNA
isolation. The DNA sources collected by blood sampling protocol described by Asiyah et al. (2016) from captive
sumatran elephant in ETC, WKNP, and be carried to laboratory in cold condition. The DNA sources stored at 4oC
and isolated following commercial protocol. The result of DNA isolation stored at -20oC until amplification
analysis. DNA isolation was successfully done, for further individual genetic ID building.


Keywords


Conservation; DNA isolation; sumatran elephant’s ID Card; WKNP

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DOI: https://doi.org/10.21157/ijtvbr.v3i1.11368

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Copyright© 2016 | ISSN: 2503-4715 

Published by:
The Faculty of Veterinary Medicine of Syiah Kuala University
In cooperation with:
Center for Tropical Veterinary Studies of Syiah Kuala University
and Indonesian Veterinary Medical Association (PDHI)


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