The Effect of Ethanol Extract of Malacca Leaves (Phyllanthus emblica) on The Number of Fibroblast Cells in White Rats (Rattus norvegicus) Burns Wound

Ajirni Ajirni, Nuzul Asmilia, Nazaruddin Nazaruddin, Amalia Sutriana, Dian Masyitha, Muhammad Isa


Burns are caused by heat exposure, such as fire, radiation, electricity or chemicals that can damage the skin and affect the body's systems. The aim of this study was to find out the effect of the ethanol extract of Malacca leaves (Phyllanthus emblica) on the number of fibroblast cells in white rats (Rattus norvegicus) that have burned. This study used 24 white rats (Rattus norvegicus) were divided into 4 groups that smeared with aquadest as a negative control (P1), 5% ethanol extract gel of Malacca leaves (P2), 10% ethanol extract gel of Malacca leaves (P3), and positive control applied with bioplasenton® gel (P4). The IIA degree burn were created by placing a 2x2 cm hot iron plate on the back of the rat for 5 seconds. The euthanasia performed to all animal and the skin samples were collected after 15 days of treatment. Then histopathological preparations were made using HE staining. The number of fibroblast cells were analyzed by ANOVA test. The average number of white rats (Rattus norvegicus) fibroblast cells that suffered burns P1 (negative control) had a number of 7 ± 1.4 cells/visual. Whereas th e P2 group had a number of 4.2 ± 1.58 cells/visual. This value has a significant difference with the negative control. But the P2 and P3 values (3 ± 1.51 cells/visual) there is no significant different with the P4 value (positive control) with an average number of P4 fibroblast cells were 2 ± 0.4 cells/visual. The results of this study concluded that the ethanol extract of malacca leaves 5% and 10% had an effect againts accelerating burns healing in white rats ( Rattus norvegicus).

Full Text:



Ambiyani, W. 2013. Pemberian salep ekstrak daun mengkudu (Morinda citrifolia L.) meningkatkan proses regenerasi jaringan luka pada tikus putih galur wistar (Rattus norvegicus) jantan. Tesis. Program Magister Program Studi Ilmu Biomedik Program Pascasarjana Universitas Udayana, Denpasar.

Barbul, A. 2005. Wound Healing. In: F.

Charles Brunicardi, Dana K., Andersen, Timothy R., Billiar, David L., et al., eds. Schwartsz’s principles of surgery. 8th ed. New York: McGraw-Hill Companies.

Corwin, E. J. 2009. Buku Saku Patofisiologi.

EGC, Jakarta.

Darby, I.A. and Hewitson, T.D. 2007.

Fibroblast differentiation in wound healing and fibrosis. International Review of Cytology, 257: 143-79.

Dhale, D.A. 2012. Pharmacognostic evaluation of Phyllanthus emblica Linn (Euphorbiaceae). Int. J. Pharm. Bio. Sci,

(3): 210-217.

Fauzi, N.I., S.A. Ardiansyah, and Hidayat.

Screening of mechanism of action of malaka leaves (Phyllanthus emblica L.) as anti-diabetic. Jurnal Tm Conference Series. 1(3): 107-111.

Hidayat, T.S.N. 2013. Role of topical extract aloe vera gel in deep burn wound healing in rat. Thesis. Airlangga University School of Medicine.

Iwan, J. and Nur, A. 2010. The comparation between topical Aplication Of the Aqueous Leaf Extract of Carica Papaya (ALEC) & khaula honey in accelerating skin wound healing in mice (Mus musculus). Majalah Kedokteran Bandung, 42(2): 76-81.

Jong, W. 2005. Buku Ajar Bedah. EGC, Jakarta.

Kiernan, J. A. 1990. Histological & Histochemical Methods: Theory and Practice. 2nded. Pergamon Press, Oxford. Laila, L., F. Febriyenti, F.M. Salhimi, and S. Baie. 2011. Wound healing effect of haruan (Channa striatus) spray. International Wound Journal, 8(5): 484-

Li, K., Y. Diao, H. Zhang, S. Wang, Z.

Zhang, B. Yu, S. Huang, H. Yang. 2011. Tannin extracts from Immature fruits of terminalia chebula fructus Retz. promote cutaneous wound healing in rats. BMC Complementary and Alternative Medicine, 11(86): 1-9.

Porter, S. 2007. The role of the fibroblast in wound contraction and healing. Wounds UK, 3(1): 33-40.

Rismana, E., R. Idah, Y. Prasetyawan, B.

Olivia, dan Y. Erna. 2013. Efektivitas Khasiat Pengobatan Luka Bakar Sediaan Gel Mengandung Fraksi Ekstrak Pegagan Berdasarkan Analisis Hidroksiorolin dan Histopatologi pada Kulit Kelinci. Penelitian Kesehatan. 41: 45-60.

Thomas. 1989. Traditional Medicinal

Plants. Kanisius, Yogyakarta.

Werner, S., T. Krieg, and H. Smola. 2007.

Keratinocyte-fibroblast interaction in wound healing. Journal of Investigative Dermatology, 127: 998-1008.

Widyantoro, O.B. and Sugihartini, N. 2015.

Evaluation of physical properties and activity test of extract of (Leucaena glauca), benth leaves in variation type of base ointment as wound. Media Farmasi,

(2): 186-198



  • There are currently no refbacks.

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Copyright© 2016 | ISSN: 2503-4715 

Published by:
The Faculty of Veterinary Medicine of Syiah Kuala University
In cooperation with:
Center for Tropical Veterinary Studies of Syiah Kuala University
and Indonesian Veterinary Medical Association (PDHI)

Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations


Creative Commons License
is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.