THE EFFECT OF MALACCA LEAVES (Phyllantus emblica) ETHANOLIC EXTRACT ON Plasmodium falciparum GROWTH IN VITRO

Nuzul Asmilia, T Armansyah TR, Dwinna Aliza, Juli Melia, Erdiansyah Rahmi, Lingga Surya Maret Daulay

Abstract


The aim of this research was to find out in vitro antiplasmodium activity of Malacca leaves (Phyllantus emblica) ethanolic extract against Plasmodium falciparum growth. In this study, Plasmodium culture contained 5% parasitemia in ring stage was cultured using candle jar method and antiplasmodial activity test was carried out using microculture. The treatments were divided into 7 groups with four repetitions. K1 as negative control group was given Roswell Park Memorial Institute (RPMI), while K2 as positive control group was given artesdiaquine. Groups K3, K4, K5, K6, and K7 group was added with 100 µg/mL, 75 µg/mL, 50 µg/mL, 25 µg/mL, and 5 µg/mL of Malacca leaves ethanolic extract, respectively. Antiplasmodial activity was determined by inhibition concentration of 50% parasite growth (IC50). The data were analyzed using ANOVA and followed by Duncan test. The average of parasitemia level in group K1, K2, K3, K4, K5, K6, and K7 were 55.25±15.62, 8.50±2.52, 8.50±3.00, 9.25±0.95, 9.00±2.70, 9.79±2.06, and 10.75±2.22, respectively. The average of inhibition percentage in group K1, K2; K3; K4; K5; K6; and K7 were 0.00±0.00%, 84.62±4.55%; 84.62±5.43%; 83.26±1.73%; 83.71±4,90%; 82.35±3,73%; and 80.54±6.83%, respectively (P<0.01). The results showed that the administration of malacca leaves ethanolic extract significantly affect (P<0.01) the inhibition of Plasmodium growth as compared to group K1 (negative control). Probit analysis reveals the IC50 value was 3.889 µg/mL. In conclusion, all doses of malacca leaves ethanolic extract used in this study was able to inhibit Plasmodium falciparum growth with IC50 value was 3.889 µg/mL.

Keywords


antiplasmodium, malaria, Phyllanthus emblica, Plasmodium falciparum

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References


Adams, Y., S.L. Smith, R.S. Albiez, and K.T. Andrews. 2005. Carragenans inhibit the in vitro growth of Plasmodium falciparum and cytoadhesiont CD36. Parasitol. Res. 97: 290-294.

Arsin, A.A. 2012. Malaria di Indonesia Tinjauan Aspek Epidemiologi. Masagena Press, Makassar.

Charoenteeraboon, J., C. Ngamkiide chakul, N. Doonthornchareonnon, K. Jaijoy, and S. Sireerata wong. 2010. Antioxidant activities of the standardized water extract from fruit of Phyllanthus emblica Linn. Songklanakarin. J. Sci. Technol. 32 (6): 599-604.

Dang, G.K., R.R. Parekar, S.K. Kamat, A.M. Scindia, and N.N. Rege. 2011. Anti- inflamatory acvtivity of Phyllanthus emblica, Plumbago zeylanica and Cyperus- rotundus in acute models of inflammation. Phytother Res. 25 (6): 904- 908.

Departemen Kesehatan RI. 2013. Laporan Riset Kesehatan Dasar (Riskesdas) 2013 Bidang Biomedis. Badan Litbangkes, Depkes RI, Jakarta.

Dhale, D.A. and U.P. Mogle. 2011. Phytochemical screening and antibacterial activity of Phyllanthus emblica Linn. Science Research Reporter. 1 (3): 138-142.

Dubar, F., T.J. Egan, B. Pradines, D. Kutter, K.K. Ncokazi, and D. Forge. 2010. The antimalaria ferroquine: role of the metal and intramolecularhydrogen bond in activity and resistance. A.C.C. Chem. Biol. 6(3): 275-287.

Harijanto. P. 2011. Tata laksana malaria untuk Indonesia. Buletin Jendela. Data dan Informasi. 1 (1): 23-27.

James, B.P., S.K. Sharma, A. Joseph, and A.J.M. Christina. 2004. Evaluation of antipyretic and analgesic activity of emblica Officinalis Gaertn. Journal of Ethnopharmacology. 95(1) : 83-85.

Kaur, J., D. Kaur, H. Singh, and M.U. Khan. 2013. Emblic a Officinalis: Ameritocratic drug for treating various disorders. Indo American Journal of Pharmaceutical Research. 4477-4496.

Kementerian Kesehatan RI. 2013. Pedoman Tata Laksana Malaria. Peraturan Menteri Kesehatan Republik Indonesia Nomor 5 Tahun 2013. Indonesia.

Khan, K.H. 2009. Roles of emblica Officinalis in medicine. Botany Research International. 2(4): 218-228.

Malar, H.L.V. and S. Mary. 2009. Hepato-protektive activity of Phyllanthus emblica against paracetamol induced hepatic damage in wister albino rats. African Journal of Basic and Applied Sciences. 1 (1-2): 21-25.

Malliga, N.E., M.S. Dhanarajan, and I. Elangovan. 2015. Evaluation of antibacterial and antifungal activity of Phyllanthus emblica leaf extract. I.R.J.P.B. 2(2): 59-66.

Ngamkitidechakul C., K. Jaijoy, P. Hansaku, N. Soonthornchareonnon, and S. Sireeratawong. 2010. Antitumour effects of Phyllanthus emblica Linn induction of cancer cell apoptosis and inhibition of in vivo tumour promotion and in vitro invasion of human cancer cells. Phytother Res. 24: 1405-1413.

Nicolis, E, I. Lampronti, M.C. Dechecchi, and Pyrogallol. 2008. An active compound from the medicinal plant Phyllanthus emblica Officinalis, regulates expression of pro inflammatory genesin bronchial epithelial cells. Int. Immunopharmacol. 8(12): 1672-1680.

Phillipson, J.D., and C.W Wright. 1991. Antiprotozoal agents from plant sources. Planta. Med. 57(7): 53-59.

Pinmai, K., W. Hiriote, N. Soonthornchareonnon, K. Jongsakul. S. Sireeratawong, and S.T. Udom. 2010. In vitro and in vivo antiplasmodial and cytotoxicity of water extracts of Phyllanthus emblica, Terminalia chebula, Terminalia bellerica. J. Med. Assoc. Thai. 93 (7): 120-126.

Pouplin, J.N., T.H. Tran, C. Dolecek, J. Farrar, P. Caron, B. Bodo, and P. Grellier. 2007. Antimalaria and cytotoxic activities of ethnopharmaco logically selected medical plants from South Vietnam. J. Of Ethnopharmacology. 104: 168-174.

Qureshi, S.A., W. Asad, and V. Sultana. 2009. The effect of Phyllanthus emblica Linn on type-II diabetes, triglycerides and liver-specific enzyme. Pakistan Journal of Nutrition. 8(2): 125-128.

Ravikumar, S., S.J. Inbaneson, and P. Suganthi. 2012. In vitro antiplasmodial activity of chosen terrestrial medicinal plants againts Plasmodium falciparum. Asian Pasific Journal of Tropical Biomedicine. 252-256.

Rinidar., M. Isa, dan T. Armansyah. 2013. Nilai inhibition concentration IC50 ekstrak metanol daun sernai (Wedelia biflora) terhadap Plasmodium falciparum yang diinkubasi selama 32 dan 72 jam. Jurnal Medika Veterinaria. 7(1): 8-12.

Rowe, A., A.R. Berendt, K. Marsh, and C.I. Newbold. 1994. Plasmodium falciparum: a family of sulphated glycoconjugates disrupts erythrocyte rosettes. Exp. Parasitol. 79: 506-516.

Singh, E., S. Sharma, A. Pareek, J. Dwivedi, S. Yadav, and S. Sharma. 2011. Phytochemistry, traditional uses and cancer chemopreventive activity of amla (Phyllanthus emblica): the sustainer. Journal of Applied Pharmaceutical Science. 2(1): 176-183.

Singh, N., C. Mathur, N.A. Sase, S. Rai, and J. Abraham. 2015. Pharmaceutical properties of emblica Officinalis and Phyllanthus emblica extracts. R.J.P.B.C.S. 6(1): 1007 1016.

Sumalatha, D. 2013. Antioxidant and antitumor activity of Phyllanthus emblica in colon cancer cell lines. Int. J. Curr. Microbiol. App. Sci. 2(5): 189-195.

Tasdemir, D., B. Topalogu, R. Perozzo, R. Brun, O. Neill, and N.M. Carballeira. 2007. Marine natural products from the Turkish sponge Agelas oroides that inhibit the enoyl reuctases from Plasmodium falciparum, Mycobacterium tuberculosis and Escherichia coli. Bioorg. Med. Chem. 15: 6834-6845.




DOI: https://doi.org/10.21157/j.ked.hewan.v12i4.10215

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