Rifky Rizkiantino, I Wayan Teguh Wibawan, Fachriyan Hasmi Pasaribu, Retno Damajanti Soejoedono, Okti Nadia Poetri, Wyanda Arnafia, Kris Damar Sasi, Dinda Reisinta


This study was conducted to explore the potential of adjuvant for the production of immunoglobulin Y (IgY) as antistreptococcosis in layer
chicken with mass production orientation. Enterococcus faecalis which causes streptococcosis in the red tilapia was selected as a candidate
antigen. The production of immunoglobulin Y (IgY) was carried out on Isa Brown layer chickens and aged around 20 weeks. Furthermore, the
chickens were grouped into four groups (A, B, C, and D groups), each consisting of three chickens based on the type of adjuvant, while two
chickens were used as a control group. Each group was treated by giving MONTANIDE™ ISA 71R VG adjuvant (A), Freund's adjuvant (B), aluminum potassium sulphate adjuvant (KAl(SO4)2∙12H2O) concentration of 50 ppm in pH 7 (C), and only antigens without adjuvant (D). Chickens were kept for 35 days and each week was checked for presence the IgY antigen in the serum and egg yolk. Booster was conducted on 14th and 28th days of maintenance. The results showed that IgY in treatment group A was detected on day 28 in the serum and day 35 in the yolk. Whereas the treatment group B could be detected on day 35 in the serum. However, the IgY was not detected in the serum and yolk in C, D, and control groups until the end of the maintenance. Based on the results, it can be concluded that the appearance of IgY in serum and yolk in a relatively fast time is obtained in the combination of Enterococcus faecalis antigen with the emulsion of water-in-oil adjuvant (SEPPICMONTANIDE™ ISA 71R VG) compared to the other types of adjuvant that use in this study.


adjuvant; Enterococcus faecalis; immunoglobulin Y (IgY); red tilapia; streptococcosis

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Arous, J.B., S. Deville, S. Norkina, V. Borisov, T. Rozhdestvenskaia, I. Rozhdestvenskiy, J. Gaucheron, dan L. Dupuis. 2013. Montanide™ ISA 71R VG: a robust and flexible adjuvant formulation for potent and stable poultry vaccines. Diambil April, 04, 2020, dari

Awate, S., L.A. Babiuk, dan G. Mutwiri. 2013. Mechanisms of action of adjuvants. Frontiers Immunology. 4(114):1-10.

Billiau, A., dan P. Matthys. 2001. Modes of action of Freund’s adjuvants in experimental models of autoimmune diseases. J. Leukoc. Biol. 70:849–860.

de Weck, A.L. 1998. Delayed-type hypersensitivity. Encylopedia of Immunology. Elsevier Ltd, New York.

Dienes, L. 1929. The technic of producing tuberculin type of sensitization with eggwhite in tuberculosis guinea pigs. J. Immunol. 17:531–538.

Gao, X., X. Zhang, L. Lin, D. Yao, J. Sun, X. Du, X. Li, dan Y. Zhang. 2016. Passive immune-protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus infections with anti-vibrio egg yolk (IgY)-encapsulated feed. Int. J. Mol. Sci. 17(723):1-10.

Guy, B. 2007. The perfect mix: recent progress in adjuvant research. Nat. Rev. Microbiol. 5:505-517.

Heegaard, P.M.H, Dedieu, N. Johnson, M.F. Le Potier, M. Mockey, F. Mulinelli, T. Vahlenkamp, M. Vascellari, dan Skall Sørensen. 2011. Adjuvants and delivery systems in veterinary vaccinology: current state and future developments. Arch. Virol. 156:183202.

Hem, S.L., dan H. HogenEsch. 2007. Relationship between physical and chemical properties of aluminum-containing adjuvants and immunopotentiation. Expert Rev. Vaccines. 6:685–698.

HogenEsch, H. 2013. Mechanism of immunopotentiation and safety of aluminum adjuvants. Frontiers in Immunology. 3(406):1–13.

Lindblad, E.B., dan L. Duroux. 2017. Mineral adjuvants. Di dalam Immunopotentiators in Modern Vaccines. Schijns V., dan D. O’Hagan (Ed). Ed ke-2. Academic Press, Massachusetts.

Pasaribu, F.H, S. Nuryati, R. Hidayat, dan A. Somantri. 2012. Teknologi pelet ikan plus Ig-Y anti Koi Herpes Virus (KHV) sebagai pengendalian wabah penyakit KHV dalam upaya peningkatan produksi ikan mas (Cyprinus carpio carpio). Diambil Agustus, 06, 2019, dari

Rizkiantino, R., Wibawan, I.W.T., Pasaribu, F.H., Soejoedono, R.D., Arnafia, W., Ulyama, V., dan Wibowo, D.B. 2020. Isolation and characterization of the Enterococcus faecalis strain isolated from red tilapia (Oreochromis hybrid) in Indonesia: a preliminary report. Journal of Survey in Fisheries Sciences. 7(1):27-42.

Shah, R.R., M. Taccone, E. Monaci, L.A. Brito, A. Bonci, D.T. O’Hagan, M.M. Amiji, dan A. Seubert. 2019. The droplet size of emulsion adjuvants has significant impact on their potency, due toe differences in immun cell-recruitment and-activation. Nature. 9:11520.

Soejoedono, R.D. 2012. Telur ayam sebagai pabrik biologis: imunoglobulin Y berkhasiat obat. Diambil Agustus, 06, 2019, dari

Wibawan, I.W.T, N.D. Kristanti, A. Zulfa, K.D. Sasi, D.A. Permatasari, M.I. Cahyono, Julianto, G. Sibit, dan W. Arnafia. 2018. Production of IgY against infectious bursal disease virus and purification of IgY from eggs by using biocompatible technique. Intern. J. Appls. Res. Vet. Med. 16(3):175-179.

Xiao-liang, L., S. Jiang-bing, dan F. Wei-huan. 2006. Protection of Carassius auratus Gibelio against infection by Aeromonas hydrophila using specific immunoglobulins from hen egg yolk. J. Zhejiang Univ. Sci. B. 7(11):922-928.



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