Lisa Purbawaning Wulandari, Anindya Hapsari


The use of Moringa oleifera as an anti-oxidant should be investigated as an alternative treatment of follicular refinement in Polycystic ovary
syndrome (PCOS) with insulin resistance. We aimed to prove the effect of Moringa oleifera leaf extract in various dosages to decrease the theca
cell thickness of PCOS female rat with insulin resistance. This study was a laboratory experimental research. Three month old Rattus norvegicus
strain Wistar rat weighing 100-130 g were divided into 5 groups (n= 8). PCOS model obtained by giving injection of testosterone propionate for
28 days, followed by metformin therapy and Moringa oleifera leaf extract at 250 and 500 mg/kg BW for 14 days. The examination of ovarium
histology showed that leaf extract Moringa oleifera 500 mg/kg BW (0.931±0.457) significantly decreased the thickness of theca cells (P<0.05)compared to the PCOS control group. The conclusion was Moringa oleifera leaf extract as an anti-oxidant proven to decrease the thickness oftheca cell of the female rat model of PCOS.


Moringa oleifera; polycystic ovary syndrome; theca cell thickness

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Adewole SO, Caxton-Martins EA, Ojewole JA. Protective effect of quercetin on the morphology of pancreatic β-cells of streptozotocin-treated diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines. 2007;4(1):64-74.

Azziz R, Carmina E, Dewailly D, Diamanti-Kandarakis E, Escobar-Morreale HF, Futterweit W, et al. The androgen excess and PCOS society criteria for the polycystic ovary syndrome: the complete task force report. Fertility and Sterility. 2009;91(2).

Fauser BC, Tarlatzis BC, Rebar RW, et al. Consensus on women’s health aspects of polycystic ovary syndrome (PCOS): the Amsterdam ESHRE/ASRM-Sponsored 3rd PCOS Consensus Workshop Group. Fertility and sterility. 2012;97(1):28-38. e25.

Homburg R. Polycystic ovary syndrome. Best Practice & Research Clinical Obstetrics & Gynaecology. 2008;22(2):261-74.

Huang Y, Sun J, Wang X, Tao X, Wang H, Tan W. Asymptomatic chronic gastritis decreases metformin tolerance in patients with type 2 diabetes. Journal of clinical pharmacy and therapeutics. 2015;40(4):461-5.

Jahan S, Munir F, Razak S, et al. Ameliorative effects of rutin against metabolic, biochemical and hormonal disturbances in polycystic ovary syndrome in rats. Journal of ovarian research. 2016;9(1):86.

Johnson B. Clinical perspectives on the health effects of Moringa oleifera: A promising adjunct for balanced nutrition and better health. KOS health publications; 2005.

Ma Y, Andrisse S, Chen Y, Childress S, Xue P, Wang Z, et al. Androgen receptor in the ovary theca cells plays a critical role in androgen-induced reproductive dysfunction.Endocrinology 2017 Jan 1; 158(1): 98–108.

Mbikay M. Therapeutic potential of Moringa oleifera leaves in chronic hyperglycemia and dyslipidemia: a review. Frontiers in pharmacology. 2012;3.

Purwanto B, Sudiana IK, Herawati L, Aksono B. MUSCLE GLUCOSE TRANSPORTER 1 (GLUT-1) EXPRESSION IN DIABETIC RAT MODELS. Folia Medica Indonesiana. 2013;49(1):21-5.

Rojas J, Chávez M, Olivar L, et al. Polycystic ovary syndrome, insulin resistance, and obesity: navigating the pathophysiologic labyrinth. International journal of reproductive medicine. 2014;2014.

Rzepczynska IJ, Foyouzi N, Piotrowski PC, Celik-Ozenci C, Cress A, Duleba AJ. Antioxidants induce apoptosis of rat ovarian theca-interstitial cells. Biology of reproduction. 2011;84(1):162-6.

Sujatha B, Patel P. Moringa Oleifera–Nature’s Gold. Imperial Journal of Interdisciplinary Research. 2017;3(5).

Touyz RM. Molecular and cellular mechanisms in vascular injury in hypertension: role of angiotensin II–editorial review. Current opinion in nephrology and hypertension. 2005;14(2):125-31.

Umayal B, Jayakody S N, Chandrasekharan N V, Wijesundera W S, Wijeyaratne C N. Polycystic ovary syndrome (PCOS) and kisspeptin – A Sri Lankan study. J Postgrad Med 2019;65:18-23.

Vendola KA, Zhou J, Adesanya OO, Weil SJ, Bondy CA. Androgens stimulate early stages of follicular growth in the primate ovary. Journal of Clinical Investigation. 1998;101(12):2622.

Wu C, Lin F, Qiu S, Jiang Z. The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention. PloS one. 2014;9(6):e99155.



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