THE EFFECT OF BLACK CUMIN (Nigella sativa) SUPPLEMENTATION THROUGH DRINKING WATER ON THE HISTOLOGY OF SMALL INTESTINE AND LARGE INTESTINE OF BROILER CHICKENS

Madi Hartono, Purnama Edy Santosa, Ratna Ermawati, Muhammad Mirandy Pratama Sirat

Abstract


This study aimed to determine the effect of black cumin (Nigella sativa) supplementation through drinking water on the histology of broiler chickens’ small intestine and large intestine. The research was conducted from April-September 2020 in a cage facility of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. This research used a completely randomized design with four treatment groups and three replications (five broilers per replication) with a total of 60 male broilers. The treatments were drinking water without Black Cumin (P0, control); drinking water with Black Cumin 36 mg/kg BW/day (P1); 72 mg/kg BW/day (P2); and 144 mg/kg BW/day (P3). Three broilers from each group were randomly necropsied at 31 days old, and samples of the small intestine (duodenum, jejunum, and ileum) and large intestine were fixed with 10% formalin solution and sent to the Lampung Disease Investigation Center for histological preparations. The observation of preparations was carried out microscopically using the Leica DM500® Binocular Microscope to accurately calculate various parameter sizes. The results were analyzed statistically with one way Analysis of Variance at significant level 5% and if proven significant, then a Tukey test was conducted. The results of this study were that the supplementation of black cumin 72 mg/kg BW/day through drinking water could significantly increase (P<0.05) 1) the average sizes of villi height and villi area of small intestine (duodenum, jejunum, ileum) and large intestine (colon) of broiler chickens; 2) the average sizes of basal villi width, villi apex width, and gland diameter of broiler duodenum; and 3) the average size of apex villi width of broiler colon. The conclusion of this study was the supplementation of black cumin at dose of 72 mg/kg BW/day through drinking water could increase the histological sizes of the small intestine and large intestine of broilers.


Keywords


broiler; histology; large intestine; Nigella sativa; small intestine

References


Abun. 2007. Pengukuran nilai kecernaan pakan yang mengandung limbah udang windu produk fermentasi pada ayam broiler. Research report. Padjajaran University, Jatinangor.

Al-Beitawi N., S.S.E. Ghousein. 2008. Effect of feeding different levels of Nigella sativa seed (black cumin) on performance, blood constituents and carcas characteristic of broiler chick. Int. J. Poult. Sci. 7:775-778.

Alfiansyah, M. 2011. Anatomi dan Pencernaan Usus Halus. http://www. sentra-edukasi.com/. Diakses tanggal 20 Agustus 2020

Alzeer. 2008. Diseases of Poultry. 12th Ed. Blackwell, New Jersey.

Asmawati. 2014. The Effect of In Ovo Feeding on Hatching Weight and Small Intestinal Tissue Development of Native Chicken. Dissertation. Hasanuddin University, Makassar.

Awad, W.A., K. Ghareeb, S. Nitclu, S. Pasteiner, S.A. Raheem, and J. Bohm. 2008. Efect of dietary inclusion of probiotic, prebiotic and symbiotic on intestinal glucose absorb'tion of broiler chickens. J. Poult. Sci. 7: 688-691.

Badan Pusat Statistik. 2019. Populasi Ayam Ras Pedaging menurut Provinsi (Ekor) Tahun 2009-2018. https://www.bps.go.id/dynamictable/2015/12/18/1034/populasi-ayam-ras-pedaging-menurut-provinsi-2009-2018.html Diakses pada tanggal 13 Agustus 2020.

Diamita, A.A. 2009. Pengaruh pemberian minyak wijen (Sesamum indicum Linn.) dengan cold press bertingkat terhadap kerusakan histologis lambung mencit (Mus musculus) yang diinduksi aspirin. Thesis. Faculty of Medical Universitas Sebelas Maret, Surakarta.

El-Dakhakhny M., N.J. Madi, N. Lambert, H.P. Ammon. 2002. Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats. J. Ethnopharmacol. 81:161-164.

Fadillah, R. 2004. Panduan Mengelola Peternakan Ayam Broiler Komersial. PT AgroMedia Pustaka, Tangerang.

Frappier, B.L. 2006. Digestive System. In: J.A. Eurell dan B.L. Frappier, Editor. Dellmann’s Texbook of Veterinary Histology. 6th Ed. Blackwell, Oxford.

Hamzah. 2013. Respon usus dan karakteristik karkas pada ayam ras pedaging dengan berat badan awal berbeda yang dipuasakan setelah menetas. Thesis. Faculty of Animal Science. Hasanuddin University, Makassar.

Harimurti,, S. dan E.S. Rahayu. 2009. Morfologi Usus Ayam Broiler yang disuplementasi dengan Probiotik Strain Tunggal dan Campuran. Agritech. 29(3): 179-183.

Hendrik. 2009. Habbatus Sauda. Pustaka Iltazam, Solo.

Ibrahim, S. 2008. Hubungan ukuran-ukuran usus halus dengan berat badan broiler. Agripet. 8(2): 42-46.

Iji, P. A., R. J. Hughes, M. Choct and D. R. Tivey. 2001. Intestinal structure and function of broiler chickens on wheat-based diets supplemented with microbial enzyme. Asian-Aust. J. Anim. Sci. 14(1):54-60.

Kabir, S.M.L. 2010. Avian colibacillosis and salmonellosis: a closer look at epidemiology, pathogenesis, diagnosis, control and public health concerns. Int. J. Environ. Res. Public Health. 7:89-114.

Mile, R.D., G.D. Butcher, P.R. Henry, R.C. Littlel. 2006. Effect of antibiotic growth promotors on broiler performance, intestinal growth parameters, and quantitative morphology. J. Poultry Sci. 85:476-485.

Ningtias, A.S. 2013. Comparison of Growth Performance of Broilers, Kampong, and Backcross3 (Gallus gallus domesticus Linnaeus, 1758) Based on Morphometri and Histological Structure of Ileum and Breast Muscle. Thesis. Fakultas Biologi Universitas Gajah Mada. Yogyakarta

Rostinawati T. 2008. Aktivitas antibakteri ekstrak etanol dan ekstrak air kelopak bunga rosela (Hibiscus sabdariffa L.) terhadap Mycobacterium tuberculosis galur Labkes-026 (Multi Drug Resisten) dan Mycobacterium tuberculosis galur H37Rv secara in vitro. Thesis. Faculty of Pharmacy, Padjadjaran University, Bandung.

Siagian, Y.A. 2016. Gambaran Histologis dan Tinggi Vili Usus Halus Bagian Ileum Ayam Ras Pedaging yang diberi Tepung Daun Kelor (Moringa oleifera) dalam ransum. Thesis. Fakultas of Animal Science Hasanuddin University, Makasar.

Smirnov, A., R. Perez, E. Amit-Romach, D. Sklan, and Z. Uni. 2005. Mucin dynamics and microbial populations in chicken small intestine changed by dietary probiotic and antibiotic growth promotor supplementation. J. of Nutrition. 135: 187-192.

Sturkie, P.D., G.C. Whittow. 2000. Sturkie’s Avian Physiology. Academic Press, Waltham.

Sugito, M.W., D.A. Astuti, E. Handharyani, Chairul. 2007. Histopatologi hati dan ginjal pada ayam broiler yang dipapar cekaman panas dan diberi ekstrak kulitbatang Jaloh (Salix tetrasperma Roxb). JITV. 12:6873.

Sun, X. 2004. Broiler performance and intestinal alterations when fed drug-free diets. Thesis. Animal and Poultry Science. Blacksburg, Virginia

Sun, X., A. McElroy, Jr. Webb, A.E.K.E. Sefton and C. Novak. 2005. Broiler performance and intestinal alterations when fed drug-free diets. J. of Poult. Sci., 84: 1294-1302.

Suprijatna, E., U. Atmomarsono, R. Kartasudjana. 2008. Ilmu Dasar Ternak Unggas. Penebar Swadaya, Jakarta.

Thippeswammy N.B., K.A. Naidu. 2005. Antioxidant potency of cumin varietiescumin, black cumin and bitter cumin on antioxidant systems. Euro Food Res Tech. 220: 472-476

Topozoda H.H., H.A. Mazloum, M. El-Dakhakhny. 1965. The antibacterial properties of Nigella sativa seeds. J. Egypt Med. 48:187-202.

Yusuf, M.S. 2014. Efektivitas Penggunaan Jintan Hitam (Nigella sativa) dalam Proses Percepatan Penyembuhan Luka Setelah Pencabutan Gigi. Thesis. Faculty of Dentistry Hasanuddin Univerity, Makassar.

Wang, J.X. and K.M. Peng. 2008. Molecular, Cellular, and Developmental Biology Developmental Morphology of the Small Intestine of African Ostrich Chicks. J. Poultry Sci. 87: 2629-2635.




DOI: https://doi.org/10.21157/j.ked.hewan.v15i3.19774

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