IDENTIFICATION OF GENETIC DIVERSITY CYTOCHROME OXIDASE SUBUNIT II (COII) MITOCHONDRIAL GENE AS GENETIC MARKER FOR ANISAKIS SPECIES IN Euthynnus affinis

Prasetyarti Utami, Niken Satuti Nur Handayani, Rony Marsyal Kunda

Abstract


ABSTRACT 

This study aimed to get specific genetic marker for Anisakis sp. identification on mackerel tuna using gene sequence cytochrome oxidase subunit II (COII) mitochondrial deoxyribonucleic acid (mtDNA) and to identify taxonomic affiliation between Anisakis sp. from Indonesia and others Anisakis sp. from GenBank database. This study started with sample collections at three fish auctions in Cilacap (Central Java), morphology classification, DNA isolation, and molecular based identification using polymerase chain reaction (PCR) and sequencing methods. Molecular based identification of Anisakis used gene amplification COII mtDNA as a cell target prior to sequence. Morphology characteristic results showed that Anisakis nematodes which infected mackerel tuna classified as type II L3 larvae. Molecular based identification showed significant result, which found 530 bp COII DNA gene fragment similar to target cell. Gene sequencing alignment results of COII Anisakis gene compared with GenBank showed 11 different nucleotide sites that can be used as genetic barcode for Indonesian Anisakis sp. This study showed that Anisakis sp. infected mackerel tuna in Java Sea is Anisakis physeteris and considered as zoonosis.

Keywords


Anisakis; COII gene; Genetic diversity; Genetic marker; Zoonosis

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References

Abe, N., Tominaga, K., Kimata, I. 2006. Usefulness of PCR-restriction fragment length polymorphism analysis of the internal transcribed spacer region of rDNA for identification of Anisakis simplex complex. Jpn. J. Infect. Dis. 59: 60-62.

Arizono, N., Yamada, M., Tegoshi, T., Yoshikawa, M. 2012. Anisakis simplex sensu stricto and Anisakis pegreffii: biological characteristics and pathogenetic potential in human anisakiasis. Foodborne Pathog. Dis. 9:517-521.

Audicana, T.M., Ansotegui, I. J., De Corres, L.F., Kennedy, M.W. 2002. Anisakis simplex: Dangerous-dead and alive? Trends Parasitol, 18:20-25.

Audicana, M.T., Kennedy, M.W. 2008. Anisakis simplex: from obscure infectious worm to inducer of immune hypersensitivity. Clin. Microbiol. Rev. 21, 360-379.

Berland, B., 1961. Nematodes from some Norwegian marine fishes. Sarsia. 2:1-50.

Cavallero, S., Nadler, S.A., Paggi, L., Barros, N.B., D’Amelio, S. 2011. Molecular characterization and phylogeny of anisakid nematodes from cetaceans from southeastern Atlantic coasts of USA, Gulf of Mexico, and Caribbean Sea. Parasitol. Res. 108, 781-792.

Cavallero, S., Ligas, A., Bruschi, F., Smelio, S.D. 2012. Molecular Identification of Anisakis spp. From Fishes Collected in the Tyrrhenian Sea (NW Mediterranean). Vet Parasitology 187: 563-566.

Chai, J.Y., Darwin Murrell, K., Lymbery, A.J. 2005. Fish-borne parasitic zoonoses: status and issues. Int J Parasitol. 35: 1233-1254.

Daulay. 2003. Prevalensi Anisakis pada Delapan Jenis Ikan Laut TPI Daerah Istimewa Yogyakarta. Tesis. Yogyakarta: Pasca Sarjana UGM.

D’Amelio, S., Mathiopoulos, K.D., Santos, C.P., Pugachev, O.N., Webb, S.C., Picanco, M., Paggi, L. 2000. Genetic markers in ribosomal DNA for the identification of members of the genus Anisakis (Nematoda: ascaridoidea) defined by polymerase-chain-reaction-based restriction fragment length polymorphism. Int. J. Parasitol. 30: 223-226.

D’Amelio, S., Cavallero, S., Dronen, N.O., Barros, N.B., Paggi, L. 2011. Two new species of Contracaecum Railliet & Henry, 1912 (Nematoda: Anisakidae), C. fagerholmi n. sp. and C. rudolphii F from the brown pelican Pelecanus occidentalis in the northern Gulf of Mexico. Syst. Parasitol. 81: 1-16.

Fossette, S., Jorgensen, M.H., Jensenc, M.V., Kiszka, J., Berube, N., Bertrand., Velyg, M. 2014. Humpback whale (Megaptera novaeangliae) post breeding dispersal and southward migration in the western Indian ocean. J. Exper. Marine. Biol and Eco. 450:6-14.

Gutiérrez Galindo, J.F., Osanz Mur, A.C., Mora Ventura, M.T. 2010. Occurrence and infection dynamics of anisakid larvae in Scomber scombrus,Trachurus trachurus, Sardina pilchardus, and Engraulis encrasicolus from Tarragona (NE Spain). Food Control 21: 1550-1555.

Hutomo, M., Burhanuddin., Hadidjaja, P. 1978. Observations on the incidence and intensity of infection of nematode larvae (famili Anisakidae) in certain marine fishes of waters around Panggang Island, Seribu Islands. Mar Res Indonesia 21: 49-60.

Indaryanto, F.R., Wardiatno, Y., dan Tiuria, R. 2015. Inventarisasi Cacing Parasitik pada Ikan Kembung di Perairan Teluk Banten dan Pelabuhan Ratu. J. Vet. vol.16. No.2: 196-203.

Kijewska, A., Dzido, J., Shukhgalter, O., Rokicki, J. 2009. Anisakid parasites of fishes caught on the African shelf. J. Parasitol. 95: 639-645.

Koinari, M., Karl, S., Elliot, A, dan Lymbery, A.J. 2013. Identification of Anisakis species (Nematoda: Anisakidae) in marine fish hosts from Papua New Guinea. Vet. Parasitol. 193: 126-133.

Kumar, S.,Tamura, K., Dudley, J., Nei, M. 2001. Molecular Evolutionary Genetics Analysis (MEGA) software version 6.0. Mol Biol Evol, 24:1596-1599.

Mattiucci, S., Nascetti, G., 2008. Advances and trends in the molecular systematics of anisakid nematodes, with implications for their evolutionary ecology and host-parasite co-evolutionary processes. Adv. Parasitol. 66: 47-148.

Mattiucci, S., Paoletti, M., Webb, S.C. 2009. Anisakis nascettii. sp. (Nematoda: Anisakidae) from beaked whales of the southern hemisphere: morphological description, genetic relationships between congeners and ecological data. Syst. Parasitol. 74: 199-217.

Mattiucci, S., Paoletti, M., Borrini, F., Palumbo, M., Macarone, R.P., Gomes, V., Casati, A., and Nascetti, G. 2011. First Molecular Identification of the Zoonotic Parasite Anisakis pegreffii (Nematoda: Anisakidae) in a Paraffin-embedded Granuloma taken from a Case of Human Intestinal Anisakiasis in Italy. BMC Inf Disease. 11:82.

Nadler, S.A., D’Amelio, S., Dailey, M.D., Paggi, L., Siu, S., Sakanari, J.A. 2005. Molecular phylogenetics and diagnosis of Anisakis, Pseudoterranova, and Contracaecum from northern Pacific marine mammals. J. Parasitol. 91, 1413-1429.

Piras, M,C., Tedde, T., Garippa, G., Virgilio, S., Sanna, D., Farjallah, S., Merella, P. 2014. Molecular and epidemiological data on Anisakis spp. (Nematoda: Anisakidae) in commercial fish caught off northern Sardinia (western Mediterranean sea). Vet. Parasitol. 203: 237-240.

Pontes, T., D’Amelio, S., Costa, G., Paggi, L., 2005. Molecular characterization of larval anisakid nematodes from marine fishes of Madeira by a PCR-based approach, with evidence for a new species. J. Parasitol. 91: 1430-1434.

Pozio, G. 2013. Integrating animal health surveillance and ford safety: the safety of Anisakis. Rev. Sci. Tech. Off. Int. Epic.32: 487-496.

Sakanari, J.A., McKerrow, J.H. 1989. Anisakiasis. Clin. Microbiol. Rev. 2: 278-284.

Santoro, M., Mattiucci, S., Paoletti, M., Liotta, A., Uberti, B. D., Galiero, G., Nascetti, G. 2010. Molecular identification and pathology of Anisakis pegreffii (Nematoda: Anisakidae) infection in the Mediterranean loggerhead sea turtle (Caretta caretta). Vet. Parasitol. 174: 65-71.

Setyobudi, E., Helmiati, S., and Soeparno. 2007. Infeksi Anisakis sp. pada Layur (Trichiurus sp.) di Pantai Selatan Kabupaten Purworejo. J. Perikanan, IX (1): 142-148.

Setyobudi, E., Jeon, C.H., Lee, C.H., Seong, K.B., Kim, J.H. 2011. Occurrence and identification of Anisakis spp. (Nematoda: Anisakidae) isolated from chum salmon (Oncorhynchus keta) in Korea. Parasitol. Res. 108: 585-592.

Siagian, F.E., Siagian, G.T.Y., Siagian, A.O. 2010. Kelainan yang Berhubungan dengan Larva Anisakis sp. Maj Ked FK UKI. Vol. XXVII, No. 3.

Sohn, W.M., Kang, J.M., Na, B.K. 2014. Molecular Analysis of Anisakis type I larvae in marine fish from three different sea areas in Korea. Korean. J. Parasitol. vol.4: 383-389.

Suadi., Helmiati, S., Widaningroem, R. 2007. Population and parasite parameter in hairtail (Trichiurus spp.) landed at PPS Cilacap. J Fish Sci 9 (2): 226-232.

Thompson, J. D., D.G. Higgins, and T.J. Gibson. 1994. CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, Position-specific gap penalties and weight matrix choice. Nucleic. Acid. Res., 22: 4673-4680.

Umehara, A., Kawakami, Y., Matsui, T., Araki, J., Uchida, A., 2006. Molecular identification of Anisakis simplex sensu stricto and Anisakis pegreffii (Nematoda: Anisakidae) from fish and cetacean in Japanese waters. Parasitol. Int. 55: 267-271.

Utami, P. 2013. Identifikasi Anisakis sp. pada Beberapa Ikan Laut di Beberapa Tempat Pelelangan Ikan (TPI) Cilacap. J. Mat Sain dan Tekno. Vol. 15, No.1, 201:21-28.

Valentini, A., Mattiucci, S., Bondanelli, P., Webb, S.C., Mignucci-Giannone, A.A., Colom-Llavina, M.M., Nascetti, G. 2006. Genetic relationships among Anisakis species (Nematoda: Anisakidae) inferred from mitochondrial cox2 sequences, and comparison with allozyme data. J. Parasitol. 92: 156-166.

Widjanarko, A. P. 2015. Identifikasi, prevalensi dan intensitas nematoda Anisakis pada beberapa ikan hasil tangkapan di pelabuhan perikanan pantai Sedang, Kabupaten Gunung Kidul. Skripsi. Fakultas Perikanan. UGM.

Yman, L. 2003. Spesifik IgE in the Diagnosis of Parasite-Induced Allergy. Allergy 59:14-17.

Zhu, X., Gasser, R.B., Podolska, M., Chilton, N.B. 19




DOI: https://doi.org/10.21157/j.ked.hewan.v11i3.2960

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