15. The Effect of Celery (Apium graveolens L.) Leaf Infusion on Blood Cholesterol Levels in White Male Rat (Rattus norvegicus) Induced by Alloxan

Sugito Sugito, Kiky Moelviani, Roslizawaty Roslizawaty, Zuhrawati NA, Rusli Rusli, T. Armansyah TR

Abstract


This study aimed to determine the effect of celery (Apium graveolens L.) leaves infusion on blood cholesterol levels in male rats (Rattus norvegicus) induced by alloxan. The sample used in this study were 25 white male rats Wistar strained aged 3-4 months with a bodyweight of 150-200 grams. The design used are were unidirectional pattern of completely randomized design with 5 treatments and 5 replications. Treatment 1 (P1) rats  were given with the standard feed. Treatment 2 (P2),rats  were induced by alloxan. Treatment 3 (P3) rats were induced by alloxan and given 2 ml of 5% celery leaf infusion therapy. Treatment 4 (P4) rats  were induced by alloxan and given 2 ml of 10% celery leaf infusion therapy. Treatment 5 (P5) rats  were induced by alloxan and given 2 ml of 15% celery leaf infusion therapy. Celery leaf infusion was given every morning and evening. Administration of  alloxan with a single dose of 150 mg / kg in  IP and celery leaf infusion therapy were given for 14 days. Rat blood cholesterol levels checked using the CHOD-PAP. Data were analyzed using ANOVA completely randomized design. Mean (± SD) of cholesterol in treatment P1, P2, P3, P4 and P5 sequence was 52.8 ± 9.09; 50.4 ± 18.53; 42.6 ± 4.51; 40.2 ± 52.8 ± 7.19 and 2.39. The results showed that the infusion of celery leaves was not showing any effect (P> 0.05) on blood cholesterol levels of white male rats induced by alloxan. Administration of celery leaves (Apium graveolens L.) infusion for 14 days with a concentration of 5%, 10% and 15% have no effect on blood cholesterol levels in male rats induced by alloxan.


Keywords


alloxan; celery leaves infusion; cholesterol; rat

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References


Depkes (Departemen Kesehatan Republik Indonesia). 1995. Materia Medika Indonesia. Jilid VI. Direktorat Jendral Pengawasan Obat dan Makanan, Jakarta.

Depkes (Departemen Kesehatan Republik Indonesia). 2009. Farmakope Indonesia. Edisi IV. Departemen Kesehatan Republik Indonesia, Jakarta.

Dewi, T.W.K. 2010. Hubungan Pemberian Ekstrak Etanol Patikan Kebo (Euphorbia hirta L.) dengan Kadar Kolesterol Serum Pada Tikus Putih yang Diinduksi Streptozotocin. Skripsi. Fakultas Kedokteran, Universitas Sebelas Maret, Surakarta.

Fahrefi, M. 2013. Pengaruh Fraksi Air Herba Seledri (Apium graveolens L.) Terhadap Kolesterol Total Mencit Putih Jantan Hiperkolesterol. Thesis. Fakultas Farmasi, Universitas Andalas, Padang.

Gaspersz. 1991. Metode Perancangan Percobaan. CV. Armico, Bandung.

Inawati. 2007. Pengaruh ekstrak daun inai (Lawsonia inermis Linn.) terhadap penurunan kadar glukosa, kolesterol total dan dan trigliserida darah mencit yang diinduksi aloksan. Jurnal Kimia Indonesia. 2(1):7-12.

Islamiyah, D. 2010. Pengaruh Pemberian Ekstrak Buah Jambu Biji (Psidium guajava L.) Terhadap Kadar Kolesterol Total, HDL, LDL dan Trigliserida Darah Tikus Putih (Rattus norvegicus) yang Diinduksi Aloksan. Skripsi. Fakultas Sains dan Teknologi, UIN Maulana Malik Ibrahim, Malang.

Jain, D.K. and R.K. Arya. 2011. Anomalies in alloxan-induced diabetic model : it is better to standardize it first. Indian J. Pharmacol. 43(1):91.

Kolarovic, J., M. Popovic, M. Mikov, R. Mitic, and L. Gvozdenovic. 2009. Protective effect of celery juice in treatments with doxorubicin. Molecules. 14:1627-1638.

Kolarovic, J., M. Popovic, J. Zlinska, S. Trivic and M. Vojnovic. 2010. Antioxidant activities of celery and parsley juices in rats treated with doxorubicin. Molecules. 15: 6193-6204.

Lalonde, B. 2015. Celery and Cholesterol. www.livestrong.com/article/471983-celery-and-cholesterol/ [19 Maret 2016].

Lazarow, A. 1952. Spontaneous recovery from alloxan diabetes in the rat. Diabetes. 1:363-372.

Li, P., J. Jia, D. Zhang, J. Xie, X. Xu and D. Wei. 2014. In vitro and in vivo antioxidant activities of a flavonoid isolated from celery (Apium graveolens L. var. dulce). Food Funct. 5:50-56.

Popovic, M., B. Kaurinovic, S. Trivic, N. Mimica-Dukic and M. Bursac. 2006. Effect of celery (Apium graveolens) extracts on some biochemical parameters of oxidative stress in mice treated with carbon tetrachloride. Phytother Res. 20:531-537.

Price, A.S. 1997. Patofisiologi. Edisi 1. EGC, Penerbit Buku Kedokteran, Jakarta.

Shivashri, C., T. Rajarajeshwari and P. Rajasekar. 2013. Hepatoprotective action of celery (Apium graveolens) leaves in acetaminophen-fed freshwater fish (Pangasius sutchi). Fish Physiol. Biochem. 39:1057-1069.

Soegondo, S. 2008. Hidup Secara Mandiri dengan Diabetes Melitus, Kencing Manis, Sakit Gula. Balai Penerbit FKUI, Jakarta.

Soewondo, P., S. Soegondo, K. Suastika, A. Pranoto, D.W. Soeatmadji dan A. Tjokroprawiro. 2010. Outcomes on control and complications of type 2 diabetic

patients in Indonesia. Med. J. Indonesia. 19:235-44.

Suwarso, E. dan D.N. Anggraeni. 2014. Efek infus daun seledri (Apium graveolens L.) terhadap kadar kolesterol. Prosiding Seminar Nasional. Fakultas Farmasi, Universitas Sumatera Utara, Medan.

Tsi, D., N.P. Das and B.K. Tan. 1995. Effects of aqueous

celery (Apium graveolens) extract on lipid parameters of rats fed a high fat diet. Planta Med. 61:18-21.

Unger, R.H. and D.W. Foster. 1992. Diabetes Mellitus. 1992. In Endocrinology. Wilson, J.D. and D.W. Foster. W.B. Sunders Company, A Division of Harcourt Brace and Company, London.




DOI: https://doi.org/10.21157/j.med.vet..v13i1.4309

DOI (PDF): https://doi.org/10.21157/j.med.vet..v13i1.4309.g10079

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