17. Oxytetracycline Activities To Staphylococcus aureus Biofilm Inhibition Of Aceh Cattle Preputium Isolate

Maryulia Dewi, Darmawi Darmawi, T. Zahrial Helmi, Erina Erina, Basri Gani, Eliawardani Eliawardani, Azhar Azhar

Abstract


The aim of this study was to see the antimicrobial activities of oxytetracycline to the inhibition of Staphylococcus aureus biofilm of Aceh’s cattle preputium isolates. The parameters measured in this study were the percentage of antimicrobial and biofilm inhibitory power. The antimicrobial activities were tested on Nutrient Broth using U-bottom polystyrene microplate 96 wells. The Biofilm stained using 1% of crystalviolet. Then, Optical Density (OD) read at λ 595 nm. The results showed that the oxytetracycline of 10%, 20% and 30% (μg / μL) had the antimicrobial abilities of 28.60% ± 13.23%; 36.30% ± 5.37%; and 57.70% ± 2.23%. In addition, The biofilm inhibitory capacity of oxytetracycline 10%, 20% and 30% (μg / μL) were 2.20% ± 8.77%; 2.70% ± 4.17%; and 3.73% ± 14.14%. Therefore, it can be concluded that the oxytetracycline  had the ability to inhibit the biofilm of Staphylococcus aureus.

Keywords


biofilm; Staphylococcus aureus; Aceh cattle preputium; oxytetracycline activity

Full Text:

PDF

References


Ardani, M., Sylvia, U.T.P., Triana, H. 2010. Efek campuran minyak atsiri daun cengkeh dan kulit batang kayu manis sebagai antiplak gigi. J Farmasi Indonesia, 21(3): 191–201.

Bjarsholt, Th., Jensen, P.O., Moser, C., Hoiby, N. 2011. Biofilm Infections. Springer-Verlag. New York.

Das, S.C. and K.N. Kapoor. 2004. Efect of growth medium on hydrophobicity of Staphylococcus epidermidis. Indian J. Med. Res. (119): 107 – 109.

Gordon, R.J, and Lowy, F.D. 2008. Pathogenesis of Methicillin-Resistant Staphylococcus aureus Infection. J Clinical Infectious Diseases (46):50-59.

Homenta, H. 2016. Infeksi biofilm bakterial. Jurnal e-Biomedik 1 (4): 1-11

Kacar, C., Kaya, S., 2014. Uterine infection in cows and effect of reproductive performance. Kafkas Univ. J Vet Fak Derg 20 (6) : 975-982.

Karmakar, A., Dua, P., Ghosh, C. 2016. Biochemical and Molecular Analysis of Staphylococcus aureus Clinical Isolates from Hospitalized Patients. Hindawi Publishing Corporation. Canadian Journal of Infectious Diseases and Medical Microbiology. 2016: 1-7.

Khadafi, M. 2012. Identifikasi S. aureus pada Preputium Kerbau Lumpur (Swamp buffalo) di Aceh Barat Daya. Skripsi. Fakultas Kedokteran Hewan. Universitas Syiah Kuala. Banda Aceh.

Madigan MT, Martinko JM, Brock TD. 2006. Brock Biology of Microorganisms. 11th Ed. Pearson Prentice Hall. New Jersey.

Nicolic, M., Vasic, S., Durdevic, J., Stevanovic, O., Comic, L. 2014. Antibacterial and Anti-biofilm Activity of Ginger (Zingiber officinate (Roscoe)) Ethanolic Extract. Laboratory of Microbiology, Department of Biology and Ecology, Faculty of Science, University of Kragujevac. Kragujevac J. Sci. (36) 129-136.

O'Neill, E., Humphreys, H., O'Gara, J.P. 2009. Carriage of both the fnbA and fnbB genes and growth at 37 degrees C promote FnBP-mediated biofilm development in meticillin-resistant Staphylococcus aureus clinical isolates. J Med Microbiol. 58 (4): 399-402.

Parsek, M.R., Singh, P.K. 2003. Bacterial biofilms: an emerging link to disease pathogenesis. J Annu. Rev. Microbiol. (57): 677-701.

Prawesthirini, S., Ferianto, A.,Supranianondo, K. 2012. Pola Resistensi Staphylococcus aureus yang Diisolasi dari Mastitis pada Sapi Perah di Wilayah Kerja KUD Argopuro Krucil Probolinggo terhadap Antibiotika. Jurnal Veterinaria Medika 5 (3): 181-186.

Sader, H.S., Fey, P.D., Fish, D.N., Limaye, A.P., Pankey, G., Rahal, J., Rybak, M.J., Snydman, D.R., Steed, L.L., Waites, K., Jones, R.N. 2009. Evaluation of vancomycin and daptomycin potency trends (MIC creep) against methicillin-resistant Staphylococcus aureus isolates collected in nine U.S. medical centers from 2002 to 2006. J Antimicrob Agents Chemother. 53(10): 4127-32.

Sarah, T., Aurélie, C., Mayram, H., Liesbeth, S., Silke, H., Petra, R., Inne, D., Tom, C. 2017. Community Composition Determines Activity of Antibiotics against Multispecies Biofilms. J Antimicrob Agents Chemother. 61(9): e00302-17.

Rahmi, Y., Darmawi, Mahdi, A., Faisal, J., Fakhrurrazi, dan Yudha, F. 2015. Identifikasi Bakteri Staphylococcus aureus Pada Preputium dan Vagina Kuda (equus caballus). Jurnal Medika Veterineria (9): 154-158.

Zhang, Y. 2007. Mechanisms of Antibiotic Resistance in the Microbial World. Baltimore, USA.

Zobel, R., S. Tkalcic. 2013. Efficacy of Ozone and other Treatment Modalities fot Retained Placenta in Dairy Cows. J Reproduction in Domestic Animals. (48): 121-125




DOI: https://doi.org/10.21157/j.med.vet..v13i1.8884

DOI (PDF): https://doi.org/10.21157/j.med.vet..v13i1.8884.g10116

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 by author and J. Med. Vet.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.