18. Phytochemistry and Antibacterial Activity Test of Ethanol Extracts of Soursop flower (Annona muricata L.) against Salmonella enteritidis

Zuraidawati Zuraidawati, Maryulia Dewi, Darmawi Darmawi, Sugito Sugito, Abdullah Hamzah, Winaruddin Winaruddin

Abstract


           The purpose of this research was to identified the compounds in ethanol extract of soursop flower and determined the antibacterial activity of the soursop flower extract on Salmonella enteritidis. The phytochemistry screening was done to identify the secondary metabolite compounds of this extract. The concentrations used of ethanol extract of soursop flower were 10%, 20%, 30%, 40%, 50%, with ampicillin 10 μg/disc as the positive control and 10% Dimethyl Sulfoxide (DMSO) as the negative control. The antibacterial activity test of soursop flower ethanol extract against Salmonella enteritidis was held in vitro using the paper disc diffusion method by measuring the diameter of the inhibition zone. The phytochemistry screening showed that the ethanol extract of soursop flower had secondary metabolites such as alkaloids, phenolic, and flavonoid. The result of the antibacterial activity test showed that there was no inhibition zone (bright zone) at various concentrations. So, it can be concluded that soursop flower ethanol extract (Annona muricata L.) contained the secondary metabolites such as alkaloids, phenolic, and flavonoid, and also this extract had no antibacterial activity against Salmonella enteritidis

Keywords


Antibacteria; Soursop flower ethanol extract; Salmonella enteritidis.

Full Text:

PDF

References


Alisantosa, B., Shivaprasad, H.L., Dhillon, A.S., Jack, O., Schaberg, D., and Bandli, D. 2000. Pathogenicity of Salmonella enteritidis phage types 4, 8 and 23 in spesific pathogen free chicks. Avian Pathol. 29(6): 583-592

Astawan, M. 2008. Sehat Dengan Buah. Dian Rakyat, Bogor.

Adam, R. 2015. Isolasi dan Identifikasi Senyawa Flavonoid dari Ekstrak Metanol Bunga Tanaman Sirsak. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Gorontalo, Gorontalo.

Barton, M.D. and W.S. Hart. 2001. Public Healt Risk: Antibiotic resistance. Asian-Aust. J. Anim. Sci.14(3):414-422.

Buck, J.D., 2004. Interraction of Salmonella enteritidis with the reproductive tract of the laying hen. Belgium: Divisi Pathologi Gent University.

Cowan, M.M. 1999. Plant products as antimicrobial agents. Clinical Microbiology Reviews. 12(4):564-582.

El-Sherbini, G.T.and Osman, S.M. 2013. Anthelmintic activity of unripe mangifera indica L. (mango) against strongyloides stercoralis. Int. J. Curr, Microbiol, App, Sci. 2(5): 401-409

Glisson, J.R. 1998. Use of antibiotic to control Salmonella in poultry. Proc. of International symposium on food-borne Salmonella in poultry, Baltimore Maryland.pp.173-175.

Jones, W.P. and Kinghorn, D.G. 2005. Extraction of Plant Secondary Metabolites: Methods in Biotechnology. In Natural Products Isolation. S. Sarker, D., S.Z. Latif, and A.I. Gray (Eds.). Vol. 20,. 2nd ed. Humana Press inc. Totowa, New Jersey.

Jiraungkoorskul, W., Sahaphong, S., Transatit, T., Kanwanrangsan, N. and Pipatshukiat, S. 2005. Eurytrema pancreaticum : the in vitro effect of praziquantel and triclabendazol on the adult fluke, exp. Parasitol.111: 172-177

Joe, W. 2012. Dahsyatnya Kasiat Sirsak. Andi Off Set, Yogyakarta.

Omwandho, C.O.A. and Kubota T. 2010. Salmonella enteric serovar enteritidis: a Mini-review of contamination routes and limitations to effective control. JARQ 44(1):7 – 16

Poernomo, S., Rumawas, I. and Sarosa, A. 1997. Infeksi Salmonella enteritidis pada ayam pedaging dari peternakan pembibit: Suatu laporan kasus, JITV 2(3): 194-197.

Poernomo, S. 2000. Training Microbiological Diagnostic. Balitvet Newsletter 15(1): 5 – 7.

Poernomo, S. 2004. Variasi tipe antigen Salmonella pullorum yang ditemukan di Indonesia dan penyebaran serotipe Salmonella pada ternak (PO). Wartazoa 14(4): 143 – 159.

Pelezar, M.J. dan Chan E.C.S. 2006. Dasar-Dasar Mikrobiologi (I). UI Press, Jakarta.

Purwatresna, E. 2012. Aktivitas Antidiabetes Ekstrak Air dan Etanol Daun Sirsak Secara In Vitro Melalui

Inhibisi Enzim α-Glukosidase. Skripsi. Fakultas Matematika dan Ilmu Pengetahuan Alam. Bogor.

Resti, P.A., Sri . W dan Iit . F. 2015. Uji Aktivitas Antibakteri Ekstrak Etanol Daun Sirsak (Annona muricata L) terhadap Salmonella typhi secara In Vitro. Skripsi. Fakultas Kedokteran. Universitas Tanjungpura.

Subroto, A. dan Saputro, H. 2006. Gempur Penyakit Dengan Sarang Semut. Penebar Swadaya, Jakarta.

Sarojini, N, Manjari, S.A., and Kanti, C.C. 2011.Phytochemical screening and anthelmintcs activity study of saraca indica leaves extracts. Int. Res. J. Pharm. 2(5): 194-197.

Wayne, P.A., 2014. Clinical and Laboratory Standars Institute. Performance Standars for Antimicrobial Susceptibility Testing; Twenty-Fourth Informational Supplement. CLSI, USA.




DOI: https://doi.org/10.21157/j.med.vet..v13i1.8885

DOI (PDF): https://doi.org/10.21157/j.med.vet..v13i1.8885.g10101

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 by author and J. Med. Vet.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License
J. Med. Vet. is licensed under a Creative Commons Attribution 4.0 International License.