Identifikasi Komponen Bioaktif dan Aktivitas Ekstrak Rumput Laut Merah (Eucheuma spinosum)

N Ira Sari, Andarini Diharmi, Santhy Wisuda Sidauruk, Febriani Melisa Sinurat

Abstract


Eucheuma spinosum is a species of red seaweed that has the potential as an antimicrobial compound due to the presence of active saponins and flavonoids.  This study was aimed to determine the bioactive and antimicrobial components of the crude extract of E.spinosum.  This study was used an experimental method, by extracting E.spinosum by maceration using methanol as a solvent. Analysis parameters were consisted of the identification of bioactive and antimicrobial components against Eschericia coli and Bacillus subtilis. The results were showed that the crude extract of obtained biaoctive components, respectively, flavonoids, alkaloids, steroids, and saponins. The results of the analysis of the crude extract of E. spinosum against Eschericia coli and Bacillus subtilis showed that the extract was able to inhibit the growth of both types of bacteria. The inhibition of E.spinosum extract against B.subtilis bacteria is classified as moderate to strong because it had an inhibition zone of 9.00-14.00 mm, and E. coli was  in the moderate category of inhibition zone 6.7-8.83 mm


Keywords


extraction;metthanol; Bacillus subtilis; Eschericia coli

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References


Akib, Nur Illiyyin, Inten Widuri Wulandari, Oleo, Suryani, and Hanari,‘Formulasi Gel Hand Sanitizer Antibakteri Kombinasi Ekstrak Rumput Laut Eucheuma Spinosum Dan Eucheuma Cotonii Asal Kepulauan’, Journal Fish Protech, 2.2 (2019), 180-88.

Al Haj, N., Nurmas, I. M., Mariana, N. S. 2009. Antibacterial activity in marine algae Eucheuma denticulatum against Staphylococcus aureus and Streptococcus pyogenes. Research Journal of Biological Science 4: 519 -524.

[AOAC] The Association of Official Analytical Chemists. 2005. Official Methods of the Analysis of the Association of Official Analytical of Chemist. Virgina (US): The Association of Analytical Chemist, Inc

Bansemir A, Blume M, Schröder S, Lindequist U (2006). Screening of cultivated seaweeds for antibacterial activity against fish pathogenic bacteria. Aquaculture 252: 79-84.

Chew, Y. L., Lim, Y. Y., Omar, M. and Khoo, K. S. 2008. Antioxidant activity of three edible seaweeds from two areas in South East Asia. LWT 41: 1067-1072

Diachanty, S. Nurjanah dan Abdullah A. 2017. Aktivitas Antioksidan Berbagai Jenis Rumput Laut Coklat Dari Perairan Kepulauan Seribu. Jurnal JPHPI. Vol, 20. No. 2.

Dotulong, V., Montolalu L. A. D. Y., dan Damongilala L. J. 2016. Potensi Anti Bakteri Rumput Laut Merah Laurencia sp. Asal Perairan Sulawesi Utara. Jurnal, Bioteknologi Kelautan dan Perikanan.

Hanapi A, A. Ghanaim Fasya, Ulfatul Mardiyah, Miftahurrahmah. 2013. Uji aktivitas antioksidan dan antibakteri ekstrak methanol alga merah Euchema spinosum dari Perairan Wongsorejo Banyuwangi. Journal alchemy, 2.2, 126-137.

Harborne JB. 1996. Metode Fitokimia: Penuntun Cara Modern Menganalisis Tumbuhan. Edisi kedua. ITB. Bandung.

Haris M. 2011. Penentuan Kadar Flavanoid Total dan Ak tivitas Antiok sidan Dari Daun De w a (Gynura pseudochina) Dengan Spek trofotometer UV-Visibel. Skripsi S1 (Tidak dipublikasikan). Universitas Andalas.

Kuda, T., Kunii, T., Goto, H., Suzuki, T. and Yano, T. 2007. Varieties of antioxidant and antibacterial properties of Ecklonia stolonifera and Ecklonia kurome products harvested and processed in the Noto peninsula, Japan. Food Chemistry 103: 900-905

Morales, G., Sierra, P., Mancilla, Parades, A., Loyola, L. A., Gallardo, O., and Borquez, J. 2003. Secondary Metabolis Krom Tour Medicinal Plants Krom Northerm Cile, Antimicrobial Activity, And Biotoxocity Against Artemia Salin. J. Cile Chem. 48(2)

Neldawati, Ratnawulan, Gusnedi. 2013. Analisis nilai absorbansi dalam penentuan kadar flavonoid untuk berbagai jenis daun tanaman obat. Journal Pillar of Physics (2): 76-83

Shalaby E.A., Sana M.M.S. (2012). Comparison of DPPH and ABTS Assay for Determining Antioxidant Potential of Water and Methanol Extract of Spirulina Platensis Indiah. Journal of Geo-Marine Sciences, 42 (5), 556-56

Simaremare, S. E. 2014. Skrining Fitokimia Ekstrak Etanol Daun Gatal (Laportea decumana (Roxb.) Wedd). Jurnal Farmasi, Vol. 11 No. 01.

Salem, WM, Galal H. and Nasr El-deen, F. 2011. Screening for antibacterial activities in some marine algae from the red sea (Hurghada, Egypt) African Journal of Microbiology Research Vol. 5(15): 2160-2167. DOI: 10.5897/AJMR11.390.

Sinurat A,A,Y., Renta,P,P., Herliany, N,E., Negara,B, PSP., Purnama, D. 2019. Uji aktivitas antibakteri ekstrak methanol rumput laut Gracilaria edulis terhadap bakteri Aeromonas hydrophila. Jurnal Enggano ( 4) 1: 105-114

Siregar, A. F., Agus S. dan Pringngenies D. 2012. Potensi Anti Bakteri Ekstrak Rumput Laut Terhadap Bakteri Penyakit Kulit Pseudomonas aeruginosa, Staphylococus epidermis, dan Micrococus letus. Jurnal Of Marine Research, 1(2) :152-160.

Shanmugam M, Mody KH. 2000. Heparinoidactive Sulphated Polisaccharides from Marine Algae as Potential Blood Anticoagulant Agents. Marine Algae and Marine Environment Discipline. Bhavnagar (IN): Central Salt & Marine Chemicals Research Institute.

Wardana, P. A., dan Tukiran. 2016. Skrining Fitokimia Antioksidan Ekstrak Kloroform Tumbuhan Gowok (Syzygium polycephalum). Seminar Nasional Kimia dan Pembelajarannya, Jurusan Kimia FMIPA Universitas Negeri Surabaya.

Wiyanto. D. B. 2010. Uji Aktivitas Antibakteri Ekstrak Rumput Laut Kappaphycus alvarezii dan Eucheuma denticullatum Terhadap Bakteri Aeromonas hydrophila dan Vibrio harveyii. Jurnal Kelautan. 3 (1)




DOI: https://doi.org/10.17969/jtipi.v14i1.18862

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