Kualitas Semen Kerbau pada Waktu Ekuilibrasi dan Inkubasi yang Berbeda dalam Larutan Hipoosmotic Swelling TEST

Yendraliza Yendraliza, Eka Yuliana, Muhammad Rodiallah, Zumarni Zumarni

Abstract


ABSTRAK. Penelitian ini bertujuan untuk menge­tahui waktu ekuilibrasi yang terbaik semen kerbau menggunakan pengencer andromed dan mengetahui nilai membran plasma utuh semen kerbau dalam larutan hipoosmotik pada waktu pemeraman yang berbeda. Penelitian ini terdiri dari dua tahap; Tahap pertama menggunakan Rancangan Acak Lengkap (RAL) dengan 3 perlakuan dan 5 ulangan. Perla­kuan terdiri dari waktu ekuilibrasi 3 jam, 4 jam dan 5 jam. Parameter yang diukur adalah motilitas, sperma hidup dan abnormalitas. Penelitian tahap kedua menggunakan Rancangan Acak Lengkap (RAL) dua faktor dengan 3 perlakuan dan 5 ulangan. Faktor pertama adalah waktu ekuilibrasi yang terdiri dari 3 jam, 4 jam dan 5 jam. Faktor kedua adalah waktu pencampuran semen dengan larutan hipoosmotik yang terdiri 15 menit, 30 menit dan 45 menit. pengencer yang digunakan Andromed(R) (Minitue Germany, 13503/0200). Param­eter yang diukur adalah persentase membran plasma utuh semen kerbau. Hasil penelitian yang didapatkan bahwa waktu ekuilibrasi terbaik adalah 4 jam dan 5 jam dengan nilai motilitas 62,8-63,7%, sperma hidup 66,7-66,8% dan persentase abnormalitas 10,5-11,2%. Waktu HOS test yang terbaik adalah 45 menit dan waktu ekuilibrasi 5 jam dengan nilai MPU (64,7 : 57,7%). Kesimpulan penelitian ini adalah waktu ekuilibrasi semen kerbau menggunakan pengencer andromed adalah 5 jam dengan waktu pemeraman dalam larutan hipoosmotik adalah 45 menit. 

 

(Semen quality of buffalo at different equilibration and incubation period in hipoosmotic solution of swelling TEST

ABSTRACT The aimed of this study was to find out the best equilibration time of buffalo semen used andromed diluents and the optimal time to test the integrity of plasma membrane of buffalo semen using a hypoosmotic swelling test (HOS test). This study consists of two stages; the first step was used a Completely Randomized Design (CRD) with 3 treatments and 5 replications. The treatments were equilibration time of 3 hours, 4 hours and 5 hours. The parameters measured were motility, live sperm, and abnormalities. The second stage of the study used a two-factor Completely Randomized Design (CRD) with 3 treatments and 5 replications. The first factor was the equilibration time of 3 hours, 4 hours and 5 hours. The second factor was the time of semen mixing with a hypoosmotic solution of 15 minutes, 30 minutes and 45 minutes. diluents used by Andromed(R) (Minitube Germany, 13503/0200). The parameters measured were the percentage of the integrity of the plasma membrane of buffalo semen. The results showed that the best equilibration time was 4 hours and 5 hours with motility values 62.8-63.7%, live sperm 66.7-66.8% and percentage abnormalities 10.5-11.2%. The best HOS test time is 45 minutes and the equilibration time is 5 hours with MPU value (64.7: 57.7%). The conclusion of the research is that the equilibration time of buffalo semen using andromed diluents was 5 hours with the mixing time in the hypoosmotic solution being 45 minutes.


Keywords


abnormalitas; membran plasma utuh; motilitas; sperma hidup; abnormality; integrity of membrane plasma; live sperma; motility

Full Text:

PDF

References


Akhter, S., Ansari, M.S., Rakha, B.A., Andrabi, S.M.H., Iqbal, S., Ullah, N., 2010. Cryopreservation of buffalo (Bubalus bubalis) semen in Bioxcell® extender. Theriogenology. 74(6): 951-955.

Andrabi, S., 2009. Factors Affecting the Quality of Cryopreserved Buffalo (Bubalus bubalis) Bull Spermatozoa. Reprod. Domes. Anim. 44(3): 552-569.

Ansari, M., Rakha, B., Akhter, S., 2017. Cryopreservation of Nili-Ravi buffalo (Bubalus bubalis) semen in AndroMed® extender; in vitro and in vivo evaluation. Reprod. Domes. Anim. 52(6): 992-997.

Bhakat, M., Mohanty, T.K., Raina, V.S., Gupta, A.K., Khan, H.M., 2011. Frozen semen production performance of Murrah buffalo bulls. Buffalo Bulletin. 30(2):157-162

[SNI] Badan Standardisasi Nasional. 2008. SNI 4869.2-2008 Semen Beku Kerbau, Standar Nasional Indonesia. Available at: https://id.scribd.com/document/345026647/SNI-4869-2-2008-Semen-Beku-Kerbau (Accessed: 10 February 2019).

Das, G.C., Das, P.K., Deori, S., Mazumdar, H., Bhattacharyya, B.N., Phookan, A., 2017. Seasonal variation in the characteristics of the swamp buffalo semen of northeast India. Buffalo Bulletin. 36(1): 215-219.

Febriani, G.D., Hamdan., Melia, J., 2014. Pengaruh waktu ekuilibrasi terhadap kualitas semen kerbau lumpur (Bubalus bubalis) setelah thawing. Jurnal Medika Veterinaria. 8(1): 1-4.

Garner, D.L., Hafez, E.S.E., 2016. Spermatozoa and Seminal Plasma. In Reproduction in Farm Animals. Baltimore, Maryland, USA: Lippincott Williams & Wilkins. Pp. 96-109.

Ghodasara, S.N., Gajbhiye, P.U., Ahlawat, A.R., Murthy, K.S., 2016. Evaluation of fresh semen quality and predicting the number of frozen semen doses in Jaffrabadi buffalo bull. Buffalo Bulletin. 35(1): 66-72

Goyal, R.L., Tuli, R.K., Georgie, G.C., Chand, D., 1996. Comparison of quality and freezability of water buffalo semen after washing or sephadex filtration. Theriogenology. 46(4): 679–686.

Hardyana, R., Arifiantini, R., Utami, D., 2013. Penentuan Waktu Optimal Pengujian Keutuhan Membran Plasma Sperma Semen Beku Sapi Menggunakan HOS TEST. In Prosiding Seminar Nasional: Peran reproduksi dalam penyelamtan dan pengembangan plasma nutfah hewan di Indonesia. Bogor. Pp. 105-109.

Holt, W., 2000. Basic aspects of frozen storage of semen. Anim. Reprod. Sci. 62(1-3): 3-22.

Jainudeen, M.R., Hafez, E.S.E., 2016. Cattle and Buffalo. In Reproduction in Farm Animals. Baltimore, Maryland, USA: Lippincott Williams & Wilkins. Pp. 157-171.

Jeyendran, R.S., van der Ven, H.H., Perez-Pelaez, M., Crabo, B.G., Zaneveld, L.J.D., 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70(1): 219-228.

Koonjaenak, S., Chanatinart, V., Aiumlamai, S., Pinyopumimintr, T., Rodriguez-Martinez, H., 2007. Seasonal variation in semen quality of swamp buffalo bulls (Bubalus bubalis) in Thailand. Asian J. Androl. 9(1): 92-101.

Kumar, P., Saini, M., Kumar, D., Balhara, A.K., Yadav, S.P., Singh, P., Yadav, P.S., 2015. Liposome-based semen extender is suitable alternative to egg yolk-based extender for cryopreservation of buffalo (Bubalus bubalis) semen. Anim. Reprod. Sci. 159: 38-45.

Kusumaningrum, D.A., Sianturi, R.G., 2017. Pengaruh media pengencer dan plasma semen sapi terhadap kualitas semen beku Kerbau (Bubalus bubalis). In Prosiding Seminar Teknologi dan Agribisnis Peternakan V: Teknologi dan Agribisnis Peternakan untuk Mendukung Ketahanan Pangan, Fakultas Peternakan Universitas, Jenderal Soedirman. Pp:368-374.

Leite, T.G., do Vale Filho, V.R., de Arruda, R.P., de Andrade, A.F.C ., Emerick, L.L., Zaffalon, F.G., Martins, J.A.M., de Andrade, V.J., 2010. Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved Gyr bull semen evaluated by CASA and flow cytometry. Anim. Reprod. Sci. 120(1-4): 31-38.

Manjunath, P., 2012. New insights into the understanding of the mechanism of sperm protection by extender components. Anim. Reprod. 9(4): 809-815.

Minitube. 2001. Certificate Andromed. Minitub Abfull und Labortechnik GmbH & Co KG. Germany. Available at: https://www.minitube.com/pdf/index/13503-0200_Leaflet-AndroMed_en_171002.pdf (Accessed: 14 February 2019).

Rizal, M., Riyadhi, M., 2017. Fertilitas semen kerbau rawa (Bubalus bubalis carabanensis) yang diencerkan dengan pengencer nira aren. Jurnal Veteriner. 17(3): 457-467.

Shah, S.A.H., Andrabi, S.M.H., Qureshi, I.Z., 2016. Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology. 4(5): 972-976.

Shahverdi, A., Rastegarnia, A., Rezaei, T., 2014. Effect of Extender and Equilibration Time on Post Thaw Motility and Chromatin Structure of Buffalo Bull (Bubalus Bubalis) Spermatozoa. Cell Journal. 16(3): 279-288.

Sharma, M., Bhat, Y., Sharma, N., Singh, A., 2018. Comparative study of seasonal variation in semen characteristics of buffalo bull. J. Entomol. Zool. Stud. 6(1):52-109.

Sieme, H., Oldenhof, H., Wolkers, W.F., 2016. Mode of action of cryoprotectants for sperm preservation. Anim. Reprod. Sci. 169: 2-5.

Singh, I., Balhara, A.K., 2016. New approaches in buffalo artificial insemination programs with special reference to India. Theriogenology. 86(1):194-199.

Steel, R.G.D, Torrie, J.H. dan B Sumantri. 1991. Prinsip dan prosedur statistika : suatu pendekatan biometrik. Gramedia Pustaka Utama. Jakarta

Tambing, S., Toelihere, M. and Yusuf, T., 2018. Optimization of Artificial Insemination Program in Buffalo. Available at: http://repository.pertanian.go.id/handle/123456789/4697 (Accessed: 26 February 2019).

Toelihere, M.R., 1985. Fisiologi reproduksi pada ternak. Angkasa. Available at: https://books.google.co.id/books/about/Fisiologi_reproduksi_pada_ternak.html?id=-v1wNAAACAAJ&redir_esc=y (Accessed: 26 February 2019).

Tuli, R.K., Singh, M., Matharoo, J.S., 1981. Effect of different equilibration times and extenders on deep freezing of buffalo semen. Theriogenology. 16(1): 99-104.

Watson, P., 2000. The causes of reduced fertility with cryopreserved semen. Anim. Reprod. Sci. 60: 481-492.

Zulyazaini, Z., Dasrul, Wahyuni, S., Akmal, M., Abdullah, M.A.N., 2016. Karakteristik semen dan komposisi kimia plasma seminalis sapi Aceh yang dipelihara di BIBD Saree Aceh Besar. Jurnal Agripet. 16(2):121-130.




DOI: https://doi.org/10.17969/agripet.v19i1.13191

Refbacks

  • There are currently no refbacks.


Copyright (c) 2019 Jurnal Agripet

License URL: http://www.jurnal.unsyiah.ac.id/agripet/about/submissions#copyrightNotice


Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2019 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.

 

Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Syiah Kuala University 
associated with Animal Scientist's Society of Indonesia (HILPI)
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: samadi177@unsyiah.ac.id & info.agripet@gmail.com


Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations