Efek Dekontaminasi Karkas Ayam Pedaging Menggunakan Asam Asetat, Asam Sitrat dan Kombinasinya Terhadap Angka Lempeng Total Campylobacter sp.

Zalhendra Eka Putra, Nurliana Nurliana, Razali Razali

Abstract


(Effect of poultry carcas decontamination by acetic acid, citric acid and its combination to total plate count of Campylobacter sp).

ABSTRACT. This research aimed to detect the total number of Campylobacter sp. on poultry carcass after decontamination by acetic acid, citric acid and combination of both. This research was conducted in the Laboratory of Veterinary Public Health Syiah Kuala University, Banda Aceh. The research was factorial completely randomized designed. Samples of poultry carcass were obtained from the Lamnyong market, Banda Aceh. Sixty poultry carcasses were divided into three groups of treatment and one group without treatment. Observation of each treatment was five time replicated at 0, 2, 4, 6 and 8 hours after immersion for ± 30 seconds in each of the decontamination material. Observation of Campylobacter sp. was done by inoculating every sample on CM0739 selective Campylobacter Blood free selective agar base and CCDA Selective supplement SR0155E. Measurements of Total Plate Count (TPC) Campylobacter sp according to Standard Plate Count (SPC). Data was variance analyzed (ANOVA) using SPSS. The results of research showed that the growing colonies of Campylobacter sp. were indicated by white colony with surrounded by black zone. Immersion of poultry carcass with acetic acid, citric acid and combination of the both and the observation time had no influence (P>0,05) to ALT Campylobacter sp. The research was concluded that carcass to Campylobacter sp. The immersion by acetic acid, citric acid and combination of both and the observation time were not reduce Campylobacter sp. in poultry carcass. 

 


Keywords


Acetic acid; Campylobacter sp.; citric acid; decontamination; poultry carcass

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References


Andriani, Darmono dan Kurniawati, W., 2007. Pengaruh Asam Asetat dan Asam Laktat Sebagai Antibakteri Terhadap Bakteri Salmonella sp. Yang Diisolasi Dari Karkas Ayam. Seminar Nasional Teknologi Peternakan Dan Veteriner, Balai Besar Penelitian Veteriner. Fakultas Farmasi Universitas Pancasila. 930 - 934

Andriani., Soedarwanto, M.S., Surachmi., Kusumaningrum, H.D., 2013. Kajian Risiko Campylobacter sp. pada ayam panggang. Jurnal Kedokteran Hewan. 7(1) : 56 : 60.

Balamurugan, S., Nattress, F.M., Baker, L.P., Dilts, B.D., 2011. Survival of Campylobacter jejuni on beef and pork under vacuum packaged and retail storage conditions:examination of the role of natural meat mikroflora on C. jejuni survival. Food Microbiol. 28: 1003-1010

Davidson, P.M and Branen, A.L., 1997. Antimicrobials in Food, 2 nd.ed. University of Idaho. Moscow, Marell Dekker. New York.

Doyle, M.P., Erickson, M.C., 2006. Reducing the carriage of foodborne pathogens in livestock and poultry. Poult Sci. (85): 960-973.

Evans, MR., Lane, W., Frost, J.A., Nylen, G., 1998. A Campylobacter outbreak associated with stir-fried food. Epidemiol Infect 12 : 275-279.

Ewen, C.D., Todd., Judy, D.G., Charles, A.B., S.M., 2009. Outbreaks where food workers have been implicated in the spread of foodborne disease. part6. transmission and survival of pathogens in the food processing and preparation environment. Poult Sci. 72(1): 202-219.

Fardiaz, S., 1992. Analisa Mikrobiologi Pangan. Raja Grafiondo Persada. Jakarta.

Flynn, O.M.J., Ian, S.B., David, A.M., 1994. Prevalence of Campylobacter sp on fresh retail chicken wings in Northern Ireland. J Food Prot. 57(4) : 334-336.

Gregory, E., Barnhart, H., Dreesen, D.W., Stern, N.J., Corn, J.L., 1997. Epidemiological study of Campylobacter spp. In broiler: source, time of colonization and prevalence. Avian Dis. 41(4): 890-898.

Hilbert, F., Scherwitzel, M., Paulsen, P., Szostak, M., 2010. Survival of Campylobacter jejuni under condition of atmospheric oxygen tension with the support of Pseudomonas spp. Appl Environ Microbiol. 76: 5911-5917.

Hwan, O.D., Pan., Youwen., Berry., Elaine., Cooley., Michael., Madrell., Robert., 2009. Escherichia coli 0157: H7 Strains Isolated From Environmental Sources Differ Significantly in Acetic Acid Resistance Compared With Human Outbreak Strains. Poult Sci. 72(3): 503-509.

Jime nez, S.M., Destefanis, P., Saisi, M.S., Tiburzi, M.C., Pirovani, M.E., 2005. Predictive model forreduction of Escherichiacoli during acetic acid decontamination of chicken skin. J.Appl. Microbiol. (99) : 829-835.

Laury, A.M., Alvarado, M.V., Nace, G., Alvarado, C.Z., Brooks, J.C., Echeverry, A., Brashears, M.M., 2009. Validation of a lactic acid and citric acid–based antimicrobial product for the reduction of Escherichia coli O157:H7 and Salmonella on beef tips and whole chicken carcasses. J. Food Protect. 72 (10) : 2208-2211.

Mayr , A.M., Lick, S., Bauer, J., Tharigen, D., Busch, U., Huber, I., 2010. Rapid Detection and Differentiation of Campylobacter jejuni, Campylobacter coli, and Campylobacter lari in Food, Using Multiplex Real Time PCR. J. Food Protect. 73(2) : 241-250

Meldrum, R.J., Tucker, D., Smith, R.M.M., Edwards, C., 2005. Survey of Salmonella and Campylobacter contamination of whole, raw poultry on retail sale in Wales in 2003. J. Food Protect 68: 1447-1449.

Morshedy, A.E.M.A., Sallam, K.I., 2009. Improving the microbial quality and shelf life of chicken carcasses by trisodium phosphate and lactic acid dipping. Int. J. Poult. Sci. 8(7) : 645-650.

Poeloengan, M. dan Noor, S.M., 2003. Isolasi Campylobacter jejuni pada daging ayam dari pasar tradisional dan supermarket. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. Bogor, Puslitbang Peternakan. 522 : 526.

Rahardjo, A.H.D., 2012. Efektivitas Jeruk Nipis dalam Menurunkan Bakteri Salmonella sp dan Escherichia Coli pada Dada Karkas Ayam Broiler. Ijas. 2: (3) : 91-94.

Rahman, MS., 1999. Handbook of Food Preservation. New York. Marcel Dekker Inc.

Rivoal, K., Ragimbeau, C., Salvat, G., Colin, P., Ernel, G., 2005. Genomic Diversity of Campylobacter coli and Campylobacter jejuni Isolates Recovered from Free-Range Broiler Farms and Comparison eith Isolates of Various Origins. App Environ Microbiol. 71(10) : 6216-6227.

Rushton, S.P., Humphrey, T.J., Shirley, M.D.F., Bull S., Jorgensen, F., 2009. Campylobacter Sp. in housed broiler chickens: a longitudinal study of risk factors. Epidemiol Infect. (137): 1099-1110.

Russell, JB., Gonzalez, D.Z., 1998. The effects of fermentation acids on bacterial growth. Adv Microb Physiol (39): 205-234..

Shao, W.F., Yang, C.J., Shih, D.Y.C., Chou, C.C., Yu, R.C., 2006. Amplified fragment length polymorphism, serotyping, and quinolone resistance of Campylobacter coli strains from chicken related samples and human in Taiwan. J. Food Prot. 69 (4) : 775-783.

Stern, N.J., Charlotte, M.P., Doyle, M.P., Chong, E.P. and A.M. Barbara., 1992. Compendium of Methods For the Microbiological Examination of Foods. Vanderzant, C and F.S F. Splittstoesser. (Eds). American Public Health Association.

Ultee, A., Gorris, L.G.M., Smid, E.J., 1998. Bacterial Activity of Carvacrol Towards the Foodborne Pathogen Bacillus cereus. J Appl Microbiol (85): 211-216.

Walpole, R.E., 1995. Pengantar Statistika. Jakarta:Gramedia Pustaka Utama.




DOI: https://doi.org/10.17969/agripet.v14i2.1873

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