Motilitas Ascaridia galli Dewasa dalam Larutan Ekstrak Etanol Biji Palem Putri (Veitchia merrillii)

Ummu Balqis, Darmawi Darmawi, Maryam Maryam, Muslina Muslina, Abdullah Hamzah, Razali Daud, Muhammad Hambal, Rinidar Rinidar, Abdul Harris, Muttaqien Muttaqien, Azhar Azhar, Eliawardani Eliawardani

Abstract


ABSTRAK. Tujuan penelitian ini untuk mengetahui motilitas Ascaridia galli dewasa dalam ekstrak etanol biji Veitchia merrillii. Ekstrak etanol V. merrillii dianalisis fitokimia. Sebanyak 16 ekor cacing A. galli dewasa dibagi kedalam empat kelompok. Cacing pada kelompok pertama adalah kelompok tanpa perlakuan. Cacing pada kelompok kedua diberi 0,6 mg/ml levamisole. Cacing pada masing-masing kelompok ketiga dan keempat diberi 50 dan 100 mg/ml crude ekstrak biji V. merrillii. Motilitas A. galli ditentukan dalam skor persentase setelah 12, 24, 36 jam dengan menggunakan kriteria: 3 (badan bergerak), 2 (hanya sebagian badan bergerak), 1 (tidak bergerak tetapi masih hidup), 0 (mati). Hasil fitokimia V. merrillii mengandung alkaloids, saponins, tannins, flavonoids, terpenoids. Ekstrak biji V. merrillii dosis 100 mg/ml secara in vitro dapat mempersingkat selama 12 jam waktu motilitas cacing A. galli dewasa. Penelitian ini mengindikasikan potensi anthelmintik berbasis herbal untuk pengendalian A. galli

 

(Motility of Ascaridia galli adult worms in vitro in ethanolic extracts of Nuts Veitchia merrillii

ABSTRACT. The purpose of this research was to know the motility of Ascaridia galli adult worms in aqueous ethanolic extracts of nuts Veitchia merrillii. The ethanolic extract of the V. merrillii was analyzed. Amount of sixteen head A. galli adult worms were divided into four groups. The first group, worms were left as un-treated normal controls. The second group, worms were treated with concentrations of 0,6 mg/ml levamisole. The third and fourth group, worms were treated with crude aqueous ethanolic extract of 50 and 100 mg/ml concentrations nuts of the V. merrillii, respectively. Motility of A. galli were determined after 12, 24, 36 hour by mean of persentage scored using the following criteria: 3 (moving whole body), 2 (moving only parts of the body), 1 (immobile but alive), and 0 (died). The result of phytochemical V. merrillii contains alkaloids, saponins, flavonoids, tannins, and terpenoids. V. merrillii nuts extract concentrations of 100 mg/ml in vitro can shorten the time motility A. galli adult worms for 12 hours. The study indicated the potential for developing herbal-based anthelmintics to control A. galli.


Keywords


veitchia merrillii; Ascaridia galli; motilitas; anthelmintik; motility; anthelmintics

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References


Abdelqader, A., Qaralla, B., Al-Ramamneh, D., Das, G., 2012, Anthelmintic Effects of Citrus Peels Ethanolic Extracts Against Ascaridia galli. Vet. Parasitol. 188(2): 78-84.

Ademola, I.O., Eloff, J.N., 2011a. Ovicidal and larvicidal activity of Cassia alata leaf acetone extract and fractions on Haemonchus contortus: in vitro studies. Pharm. Biol., 49: 539-544.

Ali, N., Shah, S.W.A., Shah, I., Ahmed, G., Ghias, M., Khan, I., 2011. Cytotoxic and anthelmintic potential of crude saponins isolated from Achillea Wilhelmsii C. Koch and Teucrium Stocksianum boiss. BMC Compl. Altern. Med., 11(106): 1-7.

Alrubaie, A.L., 2015. Effect of alcoholic extract of Curcuma longa on Ascaridia infestation affecting chickens. Indian J. Exp. Biol., 53: 452-456.

Anjali, S., Rao, A.R., 1995. Modulatory influence of arecanut on antioxidant 2(3)-tert-butyl-4-hydroxyanisole-induced hepatic detoxification system and antioxidant defense mechanism in mice. Cancer Lett., 91: 107-114.

Anthikat, R.R.N., Michael, A., Kinsalin, V.A., Ignacimuthu, S., 2014. Antifungal Activity of Areca catechu L. Int. J. Pharmaceu. Clin. Sci., 4(1):1-3.

Atawodi, S.E., Atawodi, J.C., 2009. Azadirachta indica (Neem): a plant of multiple biological and pharmacological activities. Phytochem. Rev., 8: 601-620.

Baby, A.A., Raphael, R.G., 2014. Potential antimicrobial, anthelmintic and antioxidant properties of Areca catechu L. root. Int. J. Pharm. Pharmaceut. Sci., 6(6): 486-489.

Balqis, U., Hambal, M., Darmawi., Utami, C.U., 2013. Histopathological Changes in Intestine of Chicken (Gallus domesticus) Infected Naturally by Ascaridia galli. Proceedings of The 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) In conjunction with The 2nd International Conference on Multidisciplinary Research (ICMR): 343-348, October 2-4, Banda Aceh, Indonesia.

Darmawi, Balqis, U., Tiuria, R., Hambal, M., Samadi., 2010. Purifikasi imunoglobulin yolk pada ayam yang divaksin terhadap ekskretori/sekretori stadium L3 Ascaridia galli. J. Agripet, 10 (2): 9-15.

Eguale, T., Tadesse., Dgiday, M., 2011. In vitro anthelmintic activity of crude extracts of five medicinal plants against egg-hatching and larval development of Haemonchus contortus. J. Ethnopharmacol., 137: 108-113.

Fahrimal, Y., Raflesia, R., 2002. Infestation stage of gastrointestinal Nematode in domestic chickens kept semi-intensively and traditionally. J. Med. Vet., 2(2): 114-118.

Hambal, M., Balqis, U., Darmawi, Utami, C.U., 2013. Infiltration of inflammatory cells in intestines of chickens infected naturally by Ascaridia galli. Proceedings of The 3rd Annual International Conference Syiah Kuala University (AIC Unsyiah) In conjunction with The 2nd International Conference on Multidisciplinary Research (ICMR): 362-364, October 2-4, Banda Aceh, Indonesia.

Islam, K.R., Farjana, T., Begum, N., Mondal, M.M.H., 2008. In vitro efficacy of some indigenous plants on the inhibition of development of eggs of Ascaridia galli (Digenia: Nematoda). Bangladesh J. Vet. Med., 6 (2): 159-167.

Jabar, A., Iqbal, Z., Kerboeuf, D., Muhammad, G., Khan, F.M.N., Afaq, M., 2006. Anthelmintic resistance: the state of play revisited. Life Sci., 79: 2413-2431.

Jeyathilakan, N., Murali, K., Anandaraj, A., Basith, S.A., 2011. In vitro evaluation of anthelmintic property of ethno-veterinary plant extracts against the liver fluke Fasciola gigantica. J. Parasit. Dis., 36(1): 26-30.

Jiraungkoorskul, W., Sahaphong, S., Tansatit, T., Kanwanrangsan, N., Pipatshukiat, S., 2005. Eurytrema pancreaticum: The in vitro effect of praziquantel and triclabendazole on the adult fluke. Exp. Parasitol., 111: 172-177.

Khokon, J.U., Sharifuzzaman., Sarker, E.H., Rahman, M.A., Kisku, J.J., Mustofa, M., 2014, Efficacy of Neem Leaf Extract Against Ascariasis in Indigenous Chicken. Int. J. Nat. Soc. Sci., 1: 25-30.

Lakshmi, V., Joseph, S.K., Srivastava, S., Verma, S.K., Sahoo, M.K., Dube, V., Mishra, S.K., Murthy, P.K., 2010. Antifilarial activity in vitro and in vivo of some flavonoids tested against Brugia malayi, Acta Tropica, 116: 127-133.

Meiyanto, E., Susidarti, R.A., Handayani, S., Rahmi, F., 2008. Ethanolic extract of Areca catechu seeds inhibit proliferation and induce apoptosis on MCF-7 cells. Majalah Farmasi Indonesia, 19(1): 12-19.

Monteiro, M.V., Bevilaqua, C.M., Morais, S.M., Machado, L.K., Camurça-Vasconcelos, A.L., Campello, C.C., Ribeiro, W.L., and Mde, A.M. 2011. Anthelmintic activity of Jatropha curcas L. seeds on Haemonchus contortus. Vet. Parasitol., 182: 259-263.

Saha, B.K., Abdullah-Al-Hasan, Md., Rahman, M.A., Hassan, Md.M., Begum, N., 2015. Comparative efficacy of neem leaves extract and levamisole against ascariasis in chicken. Int. J. Nat. Soc. Sci., (2): 43-48.

Sarojini, N., Manjari, S.A., Kanti. C.C., 2011. Phytochemical screening and anthelmintic activity study of Saraca indica leaves extracts. IRJP, 2(5): 194-197.

Tangalin, M.G.G., 2011. Anthelmintic effects of processed mature betel nut as dewormer to native chicken and small ruminants (sheep and goats). Int. Peer Rev. J., 1(1): 230-243.

Tiwow, D., Widdhi, B., Novel, S.K., 2013. Uji efek antelmintik ekstrak etanol biji pinang (Areca catechu) terhadap cacing Ascaris lumbricoides dan Ascaridia galli secara in vitro. Pharmacon., 2(02): 76-80.

Vafaei, A. 2013. Antioxidant and cytotoxic activities of Veitchia merrillii fruits, The 4th World Congres on Biotechnology, 23-25 Raleigh, North Carolina, USA.

Zhang, W.M., Huang, W.Y., Chen, W.X., Han, L., Zhang, H.D., 2014. Optimization of Extraction Conditions of Areca Seed Polyphenols and Evaluation of Their Antioxidant Activities. Molecules, (9): 16416-16427.




DOI: https://doi.org/10.17969/agripet.v16i1.3022

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