Pemisahan Spermatozoa Berkromosom X dan Y Kambing Boer dan Aplikasinya Melalui Inseminasi Buatan Untuk Mendapatkan Jenis Kelamin Anak Sesuai Harapan

Dasrul Dasrul, M. Aman Yaman, Zulfan Zulfan

Abstract


Separation of spermatozoa with x and y chromosome at boer goat and its application by artificial insemination for kid sex purpose

ABSTRACT. The purposes of this experiment are to investigate the separation of X and Y spermatozoa by measuring the spermatozoa quality, sex ratio between X and Y, capacity of fertility indicated by conception rate and sex ratio of goat boar kids. Samples, used in this experiment, are fresh semen from Boer goat with high quality consists of 4 group treatments with 6 replications 1) group of spermatozoa without separation (control), 2) group of spermatozoa separated by percoll gradient density centrifugation 3 levels (P1), 5 levels (P2) and swine up (P3). The observed parameters are spermatozoa quality, X and Y spermatozoa ratio, fertility’s capacity and sex ratio on the birth. Quality examination of spermatozoa and identify X and Y spermatozoa is based on the standard method of WHO. The conception rate was based on the ratio of pregnant goat after the first insemination. Data of spermatozoa quality and spermatozoa ratio were analyzed by using analisis of variance (ANOVA) and further analysis by LSD if there were differences between treatments. The results of this experiment showed that spermatozoa quality Boer goat significantly reduced (p<0,05) after separation with percoll gradient density centrifugation  and swim up. Percentage spermatozoa X after percoll gradient density centrifugation was significantly higher (P<0,05) compared to control and swim up. Meanwhile, the Y spermatozoa population was significantly higher (P<0,05) after swim up treatment compared to percoll gradient density centrifugation and control. The percentage of sex ratio (male: female) after insemination from percoll gradient density centrifugation produced more female than male. On the other hand, insemination from swim up produced more male than female. Sex ratio produced from separation of percoll gradient density centrifugation, swim up was difference from control semen (P<0,05). From this experiment, it was concluded that spermatozoa separation by percoll gradient density centrifugation and swim up can be used as one of the methods to separate   X and Y spermatozoa and further can be applied to get preferred sex animals.

Keywords


spermatozoa; goat boer; percoll gradient density centrifugation; swim up; ratio sex

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References


Anonimous. 2003. Petunjuk Penampungan, Produksi, Distribusi dan Evaluasi Semen Beku. Balai insiminasi buatan. Singosari. Malang.

Beconi, M. T., C.R. Francia, N. G. Mora and M. A. Affranchino, 1993. Efffect of natural antioxidants on frozen bovine semen preservation. Theriogenology 40: 841 - 851

Brandeis, V. T. and M.T. Manuel. 1993. Effect of four methods of sperm preparation on the motile concentration, morphology and acrosome status of recovered sperm from normal semen samples. J. Assists. Reprod Genet; Aug: 10 (6): 409 – 416

Check, M.L., C.R.A. Bollendorf, J.H. Check, W. Hourani, R. Long and M. McMonagle, 2000. Separation of sperm through a 12 layer percoll column decreases the percentage of sperm staining with quinacrine. Arch. Androl 44 (1); 47 – 50

Choudhary, R. V.K Chawala, N.D. Soni, Jayant Kumar and R.K. Vyas 2010. Oxidative stress and role of antioxidants in male infertility, Pak J Physiol 2010;6(2)

Dasrul, Susilawati, M. Adam, A. Damhoeri dan Sunarti, 2000a. Uji kualitas, Integritas membran spermatozoa kerbau lumpur hasil pemisahan dengan sentrifugasi gradien percoll dan filtrasi sephadeks. Laporan penelitian PHB VI tahun pertama. Universitas Syiah Kuala Banda Aceh.

Dasrul, Susilawati dan Susanna, 2000b. Pengaruh berbagai macam tehnik pemisahan terhadap integritas membrane spermatozoa kerbau lumpur, Prosseding Seminar Bioteknologi Reproduksi Brawijaya Universitas Malan Malang

Dasrul, 2002. Uji kualitas, Integritas membran spermatozoa kerbau lumpur hasil pemisahan dengan sentrifugasi, sentrifugasi gradien percoll dan filtrasi sephadeks. Prosseding Seminar Bioteknology Reproduksi Universitas Airlangga Surabaya.

Dasrul, 2005. Peran Senyawa Oksigen Reaktif Dalam Mekanisme Kerusakan Integritas Membran Spermatozoa Kerbau Lumpur Hasil Sentrifugasi Gradient Densitas Percoll. Disertasi Ilmu Kedokteran Pasca Sarjana Universitas Airlangga, Surabaya

De Jonge, C. 2005. Biological basis for human capacitation, Human Reproduction Update, Vol.11, No.3 pp. 205–214, 2005

Guzman, E.G., M. Ollero, M.C. Lopez, R.K. Sharma, J.g. Alvarez, A.J. Thomas and A. Agarwal. 2001. Differential production of reactive oxygen species by subsets of human spermatozoa at different stages of maturation. Hum. Reprod. Vol. 16.9. : 1922 – 1930

Hafez, E.S.E. 2004. X- and Y-Chromosome-Bearing Spermatzoa dalam Reproduction in Farm Animal, 8th ed. Lea & Febiger Philadelphia, USA pp 440 – 446

Hossefien de Lima, V.F.M., M.D.T. Ramaldo, L.H. Rodrigues, E.B. Malheiros and C.A. Moreira – Filho, 1999. Seperation of X-and Y-bearing buffalo spermatozoa by percoll density gradient centrifugation. Theriogenology. 53:479-80

Kaneko, S.,J. Yanagimachi, T. Kobayashi, and R. Lizuka, 1983. Separation of human X and Y Bearing sperm using percoll density gradien centrifugation, Fertil and Steril, 40

Mirajuddin, 1997. Pengaruh Preparasi sperma dengan metode sentrifugasi gradient densitas percoll dan swim up terhadap kualitas spermatozoa dan angka konsepsi pada kambing PE, Tesis Pascasarjana Unair Surabaya.

Safei, S. 1990. Pemisahan spermatozoa X dan Y dengan memakai Percoll. Dari hati sampai ke mata disunting oleh Jamal. Pusat Penelitian Andalas Padang.

Samardžija, M, T. Dobranić, M. Karadjole, Iva Getz, S. Vince, D. Gračner, N. Maćešić, and I. Filaković. 2006. The efficacy of gradient Percoll® on bull sperm separation for in vitro fertilization. Veterinarski Arhiv 76 (1), 37-44

Steel, R.G.D and Torrie. 1990. Prinsip dan Prosedur Statistika Suatu Pendekatan Biometrik. Alih Bahasa Bambang Sumantri. Gramedia Pustaka Utama, Jakarta.

Susilawati, 2000. Analisis membran spermatozoa sapi hasil filtrasi sephadeks dan sentrifugasi gradien densitas percoll pada proses seleksi jenis kelamin. Disertasi Pascasarjana Unair Surabaya

Zenichiro. K. Herlantien, Sarastina, 2002. Intruksi Praktis Teknologi Prosessing Semen Beku Pada Sapi, Jica - BIB Singosari, Malang.




DOI: https://doi.org/10.17969/agripet.v13i1.546

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