Peningkatan Titer Antibodi Terhadap Avian Influenza Dalam Serum Ayam Petelur yang Divaksin Dengan Vaksin Komersial

Ummu Balqis, Muhammad Hambal, Mulyadi Mulyadi, Samadi Samadi, Darmawi Darmawi

Abstract


Increasing of antibody titre against avian influenza in serum of vaccinated laying hens with commercial vaccine

ABSTRACT. The advantages of vaccination are that it reduces the risk of infection, and concurrently reduces morbidity, mortality and shedding of virus. The goal of the present study was to evaluate efficacy of Avian Influenza commercial vaccine based on humoral immunity responses of laying hens. Totally, 20 breakel silver layer hens were used in this research. The laying hens were vaccinated using Avian Influenza commercial vaccine (H5N1). Blood samples were collected from the axilaris vein (left or rigt) one time at prevaccination and repeated three times with an interval of one month at postvaccination. Antibody titres were examined using Hemaglutination Inhibition (HI). The result showed that Avian Influenza commercial vaccine (H5N1) was a good protection because the vaccine was able to trigger protective humoral immunity of laying hens indicated by increasing of antibody titre in serum of vaccinated laying hens during three months.


Keywords


Avian influenza; vaccine; antibody; laying hens

Full Text:

PDF

References


Camenisch G., Tini, M., Chilov, D., Kvietikova, I., Srinivas, V., Caro, J., Spielmann, P., Wenger, R.H., Gassmann, M., 1999. General applicability of chicken egg yolk antibodies: the performance of IgY immunoglobulins raised against the hypoxia-inducible factor 1. J. FASEB. 13: 81-88.

Carlander, D., 2002. Avian IgY antibody in vitro and in vivo. Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, Universitatis Upsaliensis, Upsala.

Direktorat Jenderal Peternakan, 2005. Manual Standar Kesehatan Hewan. Edisi Pedoman Surveilans dan Monitoring Avian Influenza di Indonesia, Departemen Pertanian, Jakarta.

Erina, 2006. Kajian Epidemiologi Penyebaran Avian Influenza Pada Pasar Unggas Tradisional di Nanggroe Aceh Darussalam. Laporan Hasil Penelitian, Departemen Pertanian, Jakarta.

Hammond, E., 2007. Some Intellectual Property Issues Related to H5N1 Influenza Viruses, Research and Vaccines. The Sunshine Project. Third World Network. http://www.sunshine-project.org/flu/patent_report.pdf [20 Juli 2008]

Hoffmann, E., Lipatov, A.S., Webby, R.J., Govorkova, E.A., Webster, R. G., 2005. Role of spesific hemagglutinin amino acids in the immunogenicity and protection of H5N1 influenza virus vaccines. Proceeding of the National Academy of Sciences of the United States of America (PNAS). 102(36): 12915 – 12920. http://www.pnas.org/cgi/reprint/0506416102v1.pdf [26 Desember 2006]

Karaca K., Swayne, D.E., Grosenbaugh, D., Bublot, M., Robles, A., Spackman, E., Nordgren, R., 2005. Immunogenicity of Fowlpox Virus Expressing the Avian Influenza Virus H5 Gene (TROVAC AIV-H5) in Cats. Clin Diagn Lab Immunol. 12(11): 1340–1342. http://cvi.asm.org/cgi/reprint/12/11/1340.pdf [26 Desember 2006]

Li, B., Peng, J., Niu, Z., Yin, X., Liu, F., 2005. Preparation of Anti-Idiotypic Antibody Against Avian Influenza Virus Subtype H9. Cellular and Molecular Immunology. 2(2): 155 – 157. http://www.cmi.ustc.edu.cn/2/2/155.pdf [26 Desember 2006]

OIE, 2000. Manual of Standards for Diagnostic Test and Vaccines. 4th ed. Office International des Epizooties, Paris. pp:216.

Rimmelzwaan, G.F., Kuiken, Van Amerongen, G., Bestebroer, T.M., Fouchier, R.A.M., Osterhaus, A.D.M.E., 2001. Pathogenesis of influenza A (H5N1) virus infection in a primate model . J. of Viro. 75(14):6687 - 6691. http://jvi.asm.org/cgi/content/full/75/14/6687?ijkey=45ab9cd965265f9ad98a95fe8abd8442dcb2990b [26 Desember 2006]

Rimmelzwaan, G.F., Van Riel, D., Baars, M., Bestebroer, T.M., Van Amerongen, G., Fouchier, R.A.M., Osterhaus, A.D.M.E., Kuiken, T., 2006. Influenza a virus (H5N1) infection in cats causes systemic disease with potential novel routes of virus spread within and between hosts. American J. of Pathology. 168:176-183. http://ajp.amjpathol.org/cgi/content/full/168/1/176 [26 Desember 2006]

Schade, R., Henklein, P., Hlinak, A., 1999. The Production of Avian (Egg Yolk) Antibodies: IgY. The Report And Recommendations of ECVAM Workshop 211,2. Reprinted with Minor Amendments from ATLA. 24: 925 - 934.

Soejoedono, R.D., Wibawan, I.W.T., Hayati, Z., 2005. Pemanfaatan Telur Ayam Sebagai Pabrik Biologis: Produksi ”Yolk Immunoglobulin” (IgY) Anti Plaque dan Diare dengan Titik Berat pada Anti Streptococcus mutans, Escherichia coli dan Salmonella enterotidis. Laporan Riset Unggulan Terpadu, Kementerian Negara Riset dan Teknologi, Jakarta.




DOI: https://doi.org/10.17969/agripet.v11i1.647

Refbacks

  • There are currently no refbacks.


Copyright (c)




Creative Commons LicenseISSN: 1411-4623E-ISSN: 2460-4534
Copyright© 2000-2016 | ISSN: 1411-4623 | EISSN: 2460-4534 
Jurnal Agripet is licensed under a Creative Commons Attribution 4.0 International License.

 

Published by: 
Animal Husbandry DepartmentThe Faculty of Agriculture, Syiah Kuala University 
associated with Animal Scientist's Society of Indonesia (HILPI). 
Jl. Tgk. Hasan Krueng Kalee No. 3, Kopelma Darussalam,
Banda Aceh, 23111, Indonesia.
Phone: +62-81383736633
Email: samadi177@yahoo.de & info.agripet@gmail.com


Online Submissions & Guidelines Editorial Policies | Contact Statistics Indexing | Citations