PENGGUNAAN 2,4 D UNTUK INISIASI KALUS JARINGAN NUCELLUS Mangifera odorata Griff. MELALUI BUDIDAYA JARINGAN

Chairani Siregar

Abstract


A

Application of 2,4 D for Callus Initiation of Mangifera odorata Griff.

Nucellar Tissue by Tissue Culture

 

Chairani Siregar

 

 

ABSTRACT

 

Application of 2,4 D for callus initiation from nucellar tissue of  Mangifera odorata through tissue culture has been done at Biotecnology laboratory, Agriculture Faculty, University of Tanjungpura, Pontianak. The aim of this reseach was to describe the 2,4 D concentration in half strength MS medium on the nucellar callus formation of  Mangifera odorata. This reseach has been conducted from April to July 2005, and Completely Randomized Design was applied, with 6 treatments and 4 replications in which each replication consist of 3 samples.The treatments level: d1= 1 ppm, d2= 1,2 ppm, d3= 1,4 ppm, d4= 1,6 ppm, d5= 1,8 ppm, d6= 2 ppm. The result showed that 1 ppm of 2,4 D gave the fastest callus formation (9,5 days), and the highest callus weight (85,95 mg).

 

 

 


Keywords


Mangifera odorata; 2,4 D; Callus; Tissue Culture

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References


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ISSN / E-ISSN1907-2686 / 2597-9108
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