Root induction of Phalaenopsis amabilis with various types and concentration of banana extract by in vitro

AISAR NOVITA, WILLY EKA PRASETYA, WAN ARFIANI BARUS

Abstract


Root induction is an important step in plant propagation in vitro. Root stimulation can occur in the presence of the hormones auxin and gibberellins which can be obtained from other plants, one of which is bananas. The research aims to determine the effect of various species and concentrations of the banana extract on Phalaenopsis amabilis root induction in vitro. The research was conducted in the Laboratory of Tissue Culture Alifa Agricultural Research Center (AARC), Medan, North Sumatera, from May until July 2020. The research used a Factorial Completely Randomized Design (CRD) with 2 factors, the first factor was the type of banana extract with 3 levels, consisting of Ambon, Raja, and Kepok. The second factor is the concentration of banana extract (C) with 4 levels, consisting of 50 g/l, 100 g/l, 150 g/l, 200 g/l. The results showed that the concentration of banana extract had a significant effect on the number of roots, but various species of bananas and the interaction of the species of bananas and concentration banana extract had no significant effect on root induction

Keywords


Phalaenopsis amabilis, banana extract, concentration, in vitro

References


Ningrum, E.F.C.; Ikhsanudin, N.R.; Rizka, R.P. and Endang, S. 2017. Perkembangan awal protocorm anggrek Phalaenopsis amabilis secara in vitro setelah penambahan zat pengatur tumbuh α-Naphtaleneacetic Acid dan Thidiazuron. J. Biosfera. 34 9-14.

Kee, Y.P.; Eun, J.H. and So, Y.P. 2011. Micropropagation of Phalaenopsis orchids via protocorms and proctorm-like bodies. Plant Embryo Culture: Methods and Protocols Methods in Molecular Biology. 7 293-306.

Isda, M.N. and Siti, F. 2014. Induksi akar pada eksplan tunas anggrek Grammatophylum scriptum var. citrinum secara in vitro pada media MS dengan penambahan NAA dan BAP. J. Biologi. 7 53-57.

Kasutjianingrat and Rudi, I. 2013. Media alternative perbanyakan in vitro anggrek bulan (Phalaenopsis amabilis). J. Agroteks. 3 148-189.

Nurfadilah.; Mukarlina and Elvi, R.P.W. 2018. Multipikasi anggrek hitam (Coelogyne pandurata Lindl) pada media Murashige Skoog (MS) dengan penambahan ekstrak pisang ambon dan Benzyl Amino Purin (BAP). J. Protobiont. 7 47-53.

Utami, E.S.W.; Sucipto, H. and Sri, W.M. 2016. Pengaruh pemberian ekstrak pisang pada media VW terhadap induksi akar dan pertumbuhan tunas Dendrobium lasianthera J.J.Sm. J. Agrotop. 6 35-42.

Yusuf, Y. and Ari, I. 2017. Pengaruh medium pupuk organik cair (POC) terhadap karakter morfologi dan jumlah tunas protokorm anggrek Vanda limbata Blume X Vanda tricolor Lindl. J.Bionature. 17 14-23.

Arti, L.T. dan Mukarlina. 2017. Multiplikasi anggrek bulan (Dendrobium sp.) dengan penambahan ekstrak taoge dan Benzyl Amino Purine (BAP) secara in vitro. J. Protoboint. 6 278-282.

Pratama, J. 2018. Modifikasi media MS dengan penambahan air kelapa untuk subkultur I anggrek Cymbidium. J. Agrium. 15 91-109.

Oratmangun, K.M.; Dingse, P. and Febby, E.K. 2017. Deskripsi jenis-jenis kontaminasi dari kultur kalus Catharanthus roseus L. Donnaman. J. FMIPA. 6 47-52.

Lestari, N.K.D. and Ni Wayan, D. 2017. Optimalisasi media organik untuk perbanyakan anggrek hitam (Coelogyne pandurata Lindl.) secara in vitro. J. Metamorfosa. 4 218-223.

Pasaribu, M.S.; Barus, W.A. and Kurnianto, H. 2011. Pengauh konsentrasi dan interval waktu pemberian POC NASA terhadap pertumbuhan dan produksi tanaman jagung manis (Zea mays saccharata Sturt). J. Agrium. 17 46-52.

Munar, A.; Alridiwirsah and Nisa, C. 2018. Utilization of various fish dung on the growth and production of lettuce (Lactuca sativa L.) in the aquaponic system. International Conference on Sustainable Agriculture and Natural Resources Management. 2 313-315.

Bakrie, A.H. 2008. Pertumbuhan vegetatif tanaman anggrek dendrobium (Dendrobium sp.) pada aplikasi zeolit sebagai campuran media tanam dan pupuk pelengkap cair. J. Zeolit Indonesia. 7 53-60.

Rineksane, I.A. and Masrukhan, S. 2015 Regenerasi anggrek Vanda tricolor pasca erupsi Merapi melalui kultur in vitro. ISBN: 978-602-73690-3-0378.

Ayu, I.W.; Rindang, D. and Hestin, Y. 2014. Pengaruh kombinasi Naphthalene Acetic Acid (NAA) Benzyl Amino Purine (BAP) dan jenis eksplan pada mikropropagasi anggrek Vanda tricolor Lindl. var. Suavis. J. Agrotrop. 4 13-18.

Hutami, S. 2018. Ulasan masalah pencoklatan pada kultur jaringan. Jurnal Agrobiogen 4 83-88.

Wu, H.C. and E.S Du Toit. 2004. Raducing oxidative browning during in vitro establishment of Protea cynaroides. Sci. Hort. 100 355-358.


Full Text: PDF

DOI: 10.24815/jn.v22i3.25261

Refbacks

  • There are currently no refbacks.